Cited 11 times in
Role of heat shock protein 47 in transdifferentiation of human tenon's fibroblasts to myofibroblasts
DC Field | Value | Language |
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dc.contributor.author | 박경수 | - |
dc.contributor.author | 성공제 | - |
dc.contributor.author | 이종복 | - |
dc.contributor.author | 한승한 | - |
dc.contributor.author | 홍사민 | - |
dc.date.accessioned | 2014-12-19T16:34:46Z | - |
dc.date.available | 2014-12-19T16:34:46Z | - |
dc.date.issued | 2012 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/89795 | - |
dc.description.abstract | BACKGROUND: Heat shock protein 47 (Hsp47) is a well-known molecular chaperone in collagen synthesis and maturation. The aim of this study is to investigate its putative role in the transdifferentiation of Tenon's fibroblasts to myofibroblasts. METHODS: Primary cultured human Tenon's fibroblasts were exposed to transforming growth factor-β1 (TGF-β1) for up to 48 hours. The mRNA levels of Hsp47 and α smooth muscle actin (αSMA) were determined by quantitative real time RT-PCR. After delivery of small interfering RNA (siRNA) molecules targeting Hsp47 into the cells, the expression of Hsp47 and αSMA proteins was determined by western immunoblotting. RESULTS: TGF-β1 increased the mRNA expressions of both Hsp47 and αSMA in human Tenon's fibroblasts, as determined by quantitative real time RT-PCR. However, it induced the protein expression of only αSMA but not Hsp47, as determined by western immunoblots. When siRNAs specific for Hsp47 were introduced into those cells, the TGF-β1-induced expression of αSMA was significantly attenuated on western immunoblots; after 48 hours of exposure to TGF-β1, the relative densities of immunobands were 11.58 for the TGF-β1 only group and 2.75 for the siRNA treatment group, compared with the no treatment control group (p < 0.001). CONCLUSIONS: Our data suggest that Hsp47 may be related to the TGF-β1-induced transdifferentiation of human Tenon's fibroblasts to myofibroblasts. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | BMC OPHTHALMOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cell Transdifferentiation/physiology* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Fibroblasts/cytology* | - |
dc.subject.MESH | Fibroblasts/metabolism | - |
dc.subject.MESH | Gene Expression Regulation* | - |
dc.subject.MESH | HSP47 Heat-Shock Proteins/physiology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Myofibroblasts/cytology* | - |
dc.subject.MESH | Myofibroblasts/metabolism | - |
dc.subject.MESH | RNA, Messenger/genetics* | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction | - |
dc.title | Role of heat shock protein 47 in transdifferentiation of human tenon's fibroblasts to myofibroblasts | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Samin Hong | - |
dc.contributor.googleauthor | Kyoungsoo Park | - |
dc.contributor.googleauthor | Jin Hyoung Kim | - |
dc.contributor.googleauthor | Sueng-Han Han | - |
dc.contributor.googleauthor | Jong Bok Lee | - |
dc.contributor.googleauthor | Gong Je Seong | - |
dc.identifier.doi | 10.1186/1471-2415-12-49 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01422 | - |
dc.contributor.localId | A01946 | - |
dc.contributor.localId | A03140 | - |
dc.contributor.localId | A04303 | - |
dc.contributor.localId | A04395 | - |
dc.relation.journalcode | J00370 | - |
dc.identifier.eissn | 1471-2415 | - |
dc.identifier.pmid | 22967132 | - |
dc.subject.keyword | Fibroblast | - |
dc.subject.keyword | Fibrosis | - |
dc.subject.keyword | Heat shock protein | - |
dc.subject.keyword | Myofibroblast | - |
dc.subject.keyword | Transforming growth factor-β | - |
dc.contributor.alternativeName | Park, Kyung Soo | - |
dc.contributor.alternativeName | Seong, Gong Je | - |
dc.contributor.alternativeName | Lee, Jong Bok | - |
dc.contributor.alternativeName | Han, Seung Han | - |
dc.contributor.alternativeName | Hong, Sa Min | - |
dc.contributor.affiliatedAuthor | Park, Kyung Soo | - |
dc.contributor.affiliatedAuthor | Seong, Gong Je | - |
dc.contributor.affiliatedAuthor | Lee, Jong Bok | - |
dc.contributor.affiliatedAuthor | Han, Seung Han | - |
dc.contributor.affiliatedAuthor | Hong, Sa Min | - |
dc.citation.volume | 12 | - |
dc.citation.startPage | 49 | - |
dc.identifier.bibliographicCitation | BMC OPHTHALMOLOGY, Vol.12 : 49, 2012 | - |
dc.identifier.rimsid | 31908 | - |
dc.type.rims | ART | - |
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