3 420

Cited 9 times in

Local Injection of Pulp Cells Enhances Wound Healing during the Initial Proliferative Phase through the Stimulation of Host Angiogenesis

Authors
 Hyungjoo Yang  ;  Sujung Shin  ;  Jhiweon Ahn  ;  YoonJeong Choi  ;  Kyung-Ho Kim  ;  Chooryung J. Chung 
Citation
 JOURNAL OF ENDODONTICS, Vol.39(6) : 788-794, 2013 
Journal Title
JOURNAL OF ENDODONTICS
ISSN
 0099-2399 
Issue Date
2013
MeSH
Animals ; Cell Culture Techniques ; Cell Proliferation ; Cell Transplantation ; Collagen/analysis ; Culture Media, Conditioned ; Dental Pulp/cytology* ; Dental Pulp/physiology ; Endothelial Cells/physiology ; Endothelium, Vascular/cytology ; Epithelial Cells/cytology ; Extracellular Matrix/metabolism ; Fibroblasts/physiology ; Granulation Tissue/cytology ; Hair Follicle/cytology ; Humans ; Keratinocytes/physiology ; Male ; Mice ; Mice, Inbred ICR ; Neovascularization, Physiologic/physiology* ; Paracrine Communication/physiology ; Platelet Endothelial Cell Adhesion Molecule-1/analysis ; Skin/cytology ; Vascular Endothelial Growth Factor A/analysis ; Wound Healing/physiology*
Keywords
Paracrine effect ; pulp stromal cell ; wound healing
Abstract
INTRODUCTION:
The aim of this study was to examine the effect of pulp cell injection on host angiogenesis during wound healing.
METHODS:
Pulp cells were isolated from extracted premolars by the outgrowth method. Fluorescently labeled pulp cells or phosphate-buffered saline were locally injected into a mouse wound healing model. Wound healing was evaluated using photographs, histology, and real-time reverse-transcription polymerase chain reaction. Injected cells were traced. Angiogenesis was measured by performing immunohistochemical staining of CD31, a marker of vascular endothelial cells. The level of secreted vascular endothelial growth factor in the pulp cell conditioned medium (CM) was compared with the CM of fibroblasts and keratinocytes. The paracrine effect of pulp CM on angiogenesis was evaluated by tubular network formation using endothelial cells.
RESULTS:
The local injection of pulp cells enhanced wound closure during the initial stage when compared to the injection of phosphate-buffered saline. The amount of extracellular matrix production and the expression of CD31+ cells were also increased in response to pulp cell injection when compared with the injection of phosphate-buffered saline. The fluorescently labeled pulp cells were engrafted into the hair follicles of the adjacent normal dermis but not into the wound site per se. A significantly higher level of vascular endothelial growth factor was secreted into the CM of pulp cells when compared with dermal fibroblast and keratinocytes. Tubular network formation of endothelial cells and the proliferation of dermal fibroblasts were significantly enhanced by the application of pulp cell CM when compared with control media.
CONCLUSIONS:
Our results show that local injection of pulp cells is effective in enhancing wound healing during the initial proliferative phase, especially through paracrine mechanisms regulating host angiogenesis and proliferation.
Full Text
http://www.sciencedirect.com/science/article/pii/S0099239913001398
DOI
10.1016/j.joen.2013.01.011
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Orthodontics (교정과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Ho(김경호) ORCID logo https://orcid.org/0000-0002-8154-2041
Shin, Su Jung(신수정) ORCID logo https://orcid.org/0000-0001-5795-3919
Chung, Chooryung J.(정주령) ORCID logo https://orcid.org/0000-0001-9399-7193
Choi, Yoon Jeong(최윤정) ORCID logo https://orcid.org/0000-0003-0781-8836
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87243
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links