3 196

Cited 0 times in

Hyaluronic acid receptor-targetable imidazolized nanovectors for induction of gastric cancer cell death by RNA interference

Authors
 Eunjung Kim  ;  Jaemoon Yang  ;  Hyun-Ouk Kim  ;  Yonghee An  ;  Eun-Kyung Lim  ;  Gyudo Lee  ;  Taeyun Kwon  ;  Jae-Ho Cheong  ;  Jin-Suck Suh  ;  Yong-Min Huh  ;  Seungjoo Haam 
Citation
 Biomaterials, Vol.34(17) : 4327-4338, 2013 
Journal Title
 Biomaterials 
ISSN
 0142-9612 
Issue Date
2013
Abstract
We have developed a nanovector consisting of hyaluronic acid (HA) and poly-l-lysine-graft-imidazole (PLI)-based polyplexes containing Bcl-xL-specific shRNA-encoding plasmid DNA (HA/PLI/pDNA) for CD44 targeted gastric cancer therapy. The prepared ternary polyplexes have a negative surface charge of −24 mV and a size of approximately 100 nm at an N/P ratio of 5 with HA/PLI molar ratio of 0.03. Gel electrophoresis and cell viability experiments demonstrated that the ternary polyplexes showed high stability and no cytotoxicity due to the anchored HA molecules on the surface of PLI/pDNA binary polyplexes. Selective cancer cell death was achieved by CD44-mediated gene delivery and the internalized gene was effectively escaped from endosomes due to the buffering capacity of imidazole groups in an acidic environment. These nanovectors may be highly efficient gene delivery tools that allow the selective destruction of metastatic gastric cancer cells.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86812
DOI
10.1016/j.biomaterials.2013.02.006
Appears in Collections:
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Institute for Medical Convergence (연의-생공연 메디컬융합연구소)
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Yonsei Integrative Research Institute for Cerebral & Cardiovascular Disease (뇌심혈관질환융합연구사업단)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실)
Yonsei Authors
서진석(Suh, Jin Suck) ; 양재문(Yang, Jae Moon) ; 임은경(Lim, Eun Kyung) ; 정재호(Cheong, Jae Ho) ; 허용민(Huh, Yong Min)
사서에게 알리기
  feedback
Full Text
http://www.sciencedirect.com/science/article/pii/S0142961213001750
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse