7 313

Cited 11 times in

Changes in the biological characteristics of glioma cancer stem cells after serial in vivo subtransplantation.

Authors
 Ga-Yeong Shin  ;  Jin-Kyoung Shim  ;  Ji-Hyun Lee  ;  Hye-Jin Shin  ;  Su-Jae Lee  ;  Yong-Min Huh  ;  Eui-Hyun Kim  ;  Eun-Kyung Park  ;  Se-Hoon Kim  ;  Jong Hee Chang  ;  Dong-Seok Kim  ;  Yong-Kil Hong  ;  Sun Ho Kim  ;  Seok-Gu Kang  ;  Frederick F. Lang 
Citation
 CHILDS NERVOUS SYSTEM, Vol.29(1) : 55-64, 2013 
Journal Title
 CHILDS NERVOUS SYSTEM 
ISSN
 0256-7040 
Issue Date
2013
MeSH
AC133 Antigen ; Animals ; Antigens, CD/metabolism ; Brain Neoplasms/pathology* ; Cell Cycle/physiology ; Disease Models, Animal ; Flow Cytometry ; Gene Expression Regulation, Neoplastic/physiology* ; Glioblastoma/pathology* ; Glycoproteins/metabolism ; Humans ; In Situ Nick-End Labeling ; Intermediate Filament Proteins/metabolism ; Membrane Glycoproteins/metabolism ; Mice ; Mice, Nude ; Neoplasm Transplantation/methods ; Neoplastic Stem Cells/physiology* ; Nerve Tissue Proteins/metabolism ; Nestin ; Peptides/metabolism ; Proliferating Cell Nuclear Antigen/metabolism ; Time Factors ; Transplantation, Heterologous/methods ; Tumor Cells, Cultured ; Xenograft Model Antitumor Assays/methods
Keywords
Glioma ; Glioma cancer stem cell ; Stem cell niche ; Serial subtransplantation
Abstract
PURPOSE: Currently, the interaction between the niche and glioma cancer stem cells (gCSCs) is gaining attention. However, there are few studies concerned with the effects of repeated exposure to a new microenvironment on gCSCs characteristics. In this study, serial in vivo subtransplantation was performed to create a new microenvironment. We evaluated and compared the biological characteristics of gCSCs after serial in vivo subtransplantation. METHODS: We cultured gCSCs from human glioma specimens according to cultured gliomasphere methods. The isolated gCSCs were termed zero-generation gCSCs (G0-gCSCs). By subsequent serial subtransplantation, we obtained first-generation gCSCs (G1-gCSCs) and second-generation gCSCs (G2-gCSCs). We evaluated and compared the biological characteristics of G0-gCSCs, G1-gCSCs, and G2-gCSCs. The in vitro characteristics included the morphology, surface marker profiles, and neural differentiation capacity and the in vivo characteristics was the survival of mice xenografts. Additionally, brain sections were analyzed using PCNA, TUNEL, and CD31 staining. RESULTS: We observed no significant differences in the in vitro characteristics of G0-gCSCs, G1-gCSCs, and G2-gCSCs. However, the survival time of mice glioma xenografts was significantly decreased upon serial subtransplantation. In addition, immunohistochemical analyses showed that the number of TUNEL(+) cells was significantly decreased while the number of CD31(+) cells was significantly increased with serial in vivo subtransplantation. CONCLUSIONS: There were significant in vivo biological changes in gCSCs upon serial in vivo subtransplantation, which were shorter xenograft survival, increased angiogenesis, and decreased apoptosis. This study suggests that the repeated exposure to new microenvironments may affect the biological changes in gCSCs in vivo.
Full Text
http://link.springer.com/article/10.1007%2Fs00381-012-1963-x
DOI
10.1007/s00381-012-1963-x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kang, Seok Gu(강석구) ORCID logo https://orcid.org/0000-0001-5676-2037
Kim, Dong Seok(김동석)
Kim, Sun Ho(김선호) ORCID logo https://orcid.org/0000-0003-0970-3848
Kim, Se Hoon(김세훈) ORCID logo https://orcid.org/0000-0001-7516-7372
Kim, Eui Hyun(김의현) ORCID logo https://orcid.org/0000-0002-2523-7122
Park, Eun Kyung(박은경)
Lee, Ji Hyun(이지현) ORCID logo https://orcid.org/0000-0002-9223-9478
Chang, Jong Hee(장종희)
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86169
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse