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Changes in the biological characteristics of glioma cancer stem cells after serial in vivo subtransplantation.

Authors
 Ga-Yeong Shin  ;  Jin-Kyoung Shim  ;  Ji-Hyun Lee  ;  Hye-Jin Shin  ;  Su-Jae Lee  ;  Yong-Min Huh  ;  Eui-Hyun Kim  ;  Eun-Kyung Park  ;  Se-Hoon Kim  ;  Jong Hee Chang  ;  Dong-Seok Kim  ;  Yong-Kil Hong  ;  Sun Ho Kim  ;  Seok-Gu Kang  ;  Frederick F. Lang 
Citation
 Child's Nervous System, Vol.29(1) : 55-64, 2013 
Journal Title
 Child's Nervous System 
ISSN
 0256-7040 
Issue Date
2013
Abstract
PURPOSE: Currently, the interaction between the niche and glioma cancer stem cells (gCSCs) is gaining attention. However, there are few studies concerned with the effects of repeated exposure to a new microenvironment on gCSCs characteristics. In this study, serial in vivo subtransplantation was performed to create a new microenvironment. We evaluated and compared the biological characteristics of gCSCs after serial in vivo subtransplantation. METHODS: We cultured gCSCs from human glioma specimens according to cultured gliomasphere methods. The isolated gCSCs were termed zero-generation gCSCs (G0-gCSCs). By subsequent serial subtransplantation, we obtained first-generation gCSCs (G1-gCSCs) and second-generation gCSCs (G2-gCSCs). We evaluated and compared the biological characteristics of G0-gCSCs, G1-gCSCs, and G2-gCSCs. The in vitro characteristics included the morphology, surface marker profiles, and neural differentiation capacity and the in vivo characteristics was the survival of mice xenografts. Additionally, brain sections were analyzed using PCNA, TUNEL, and CD31 staining. RESULTS: We observed no significant differences in the in vitro characteristics of G0-gCSCs, G1-gCSCs, and G2-gCSCs. However, the survival time of mice glioma xenografts was significantly decreased upon serial subtransplantation. In addition, immunohistochemical analyses showed that the number of TUNEL(+) cells was significantly decreased while the number of CD31(+) cells was significantly increased with serial in vivo subtransplantation. CONCLUSIONS: There were significant in vivo biological changes in gCSCs upon serial in vivo subtransplantation, which were shorter xenograft survival, increased angiogenesis, and decreased apoptosis. This study suggests that the repeated exposure to new microenvironments may affect the biological changes in gCSCs in vivo.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86169
Full Text
http://link.springer.com/article/10.1007%2Fs00381-012-1963-x
DOI
10.1007/s00381-012-1963-x
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
Yonsei Authors
강석구(Kang, Seok Gu) ORCID logo https://orcid.org/0000-0001-5676-2037
김동석(Kim, Dong Seok)
김선호(Kim, Sun Ho)
김세훈(Kim, Se Hoon)
김의현(Kim, Eui Hyun)
박은경(Park, Eun Kyung)
이지현(Lee, Ji Hyun)
장종희(Chang, Jong Hee)
허용민(Huh, Yong Min)
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