Cited 11 times in
Changes in the biological characteristics of glioma cancer stem cells after serial in vivo subtransplantation.
DC Field | Value | Language |
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dc.contributor.author | 강석구 | - |
dc.contributor.author | 김동석 | - |
dc.contributor.author | 김선호 | - |
dc.contributor.author | 김세훈 | - |
dc.contributor.author | 김의현 | - |
dc.contributor.author | 박은경 | - |
dc.contributor.author | 이지현 | - |
dc.contributor.author | 장종희 | - |
dc.contributor.author | 허용민 | - |
dc.date.accessioned | 2014-12-18T08:21:53Z | - |
dc.date.available | 2014-12-18T08:21:53Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0256-7040 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/86169 | - |
dc.description.abstract | PURPOSE: Currently, the interaction between the niche and glioma cancer stem cells (gCSCs) is gaining attention. However, there are few studies concerned with the effects of repeated exposure to a new microenvironment on gCSCs characteristics. In this study, serial in vivo subtransplantation was performed to create a new microenvironment. We evaluated and compared the biological characteristics of gCSCs after serial in vivo subtransplantation. METHODS: We cultured gCSCs from human glioma specimens according to cultured gliomasphere methods. The isolated gCSCs were termed zero-generation gCSCs (G0-gCSCs). By subsequent serial subtransplantation, we obtained first-generation gCSCs (G1-gCSCs) and second-generation gCSCs (G2-gCSCs). We evaluated and compared the biological characteristics of G0-gCSCs, G1-gCSCs, and G2-gCSCs. The in vitro characteristics included the morphology, surface marker profiles, and neural differentiation capacity and the in vivo characteristics was the survival of mice xenografts. Additionally, brain sections were analyzed using PCNA, TUNEL, and CD31 staining. RESULTS: We observed no significant differences in the in vitro characteristics of G0-gCSCs, G1-gCSCs, and G2-gCSCs. However, the survival time of mice glioma xenografts was significantly decreased upon serial subtransplantation. In addition, immunohistochemical analyses showed that the number of TUNEL(+) cells was significantly decreased while the number of CD31(+) cells was significantly increased with serial in vivo subtransplantation. CONCLUSIONS: There were significant in vivo biological changes in gCSCs upon serial in vivo subtransplantation, which were shorter xenograft survival, increased angiogenesis, and decreased apoptosis. This study suggests that the repeated exposure to new microenvironments may affect the biological changes in gCSCs in vivo. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | CHILDS NERVOUS SYSTEM | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | AC133 Antigen | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigens, CD/metabolism | - |
dc.subject.MESH | Brain Neoplasms/pathology* | - |
dc.subject.MESH | Cell Cycle/physiology | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | Gene Expression Regulation, Neoplastic/physiology* | - |
dc.subject.MESH | Glioblastoma/pathology* | - |
dc.subject.MESH | Glycoproteins/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | In Situ Nick-End Labeling | - |
dc.subject.MESH | Intermediate Filament Proteins/metabolism | - |
dc.subject.MESH | Membrane Glycoproteins/metabolism | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Nude | - |
dc.subject.MESH | Neoplasm Transplantation/methods | - |
dc.subject.MESH | Neoplastic Stem Cells/physiology* | - |
dc.subject.MESH | Nerve Tissue Proteins/metabolism | - |
dc.subject.MESH | Nestin | - |
dc.subject.MESH | Peptides/metabolism | - |
dc.subject.MESH | Proliferating Cell Nuclear Antigen/metabolism | - |
dc.subject.MESH | Time Factors | - |
dc.subject.MESH | Transplantation, Heterologous/methods | - |
dc.subject.MESH | Tumor Cells, Cultured | - |
dc.subject.MESH | Xenograft Model Antitumor Assays/methods | - |
dc.title | Changes in the biological characteristics of glioma cancer stem cells after serial in vivo subtransplantation. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurosurgery (신경외과학) | - |
dc.contributor.googleauthor | Ga-Yeong Shin | - |
dc.contributor.googleauthor | Jin-Kyoung Shim | - |
dc.contributor.googleauthor | Ji-Hyun Lee | - |
dc.contributor.googleauthor | Hye-Jin Shin | - |
dc.contributor.googleauthor | Su-Jae Lee | - |
dc.contributor.googleauthor | Yong-Min Huh | - |
dc.contributor.googleauthor | Eui-Hyun Kim | - |
dc.contributor.googleauthor | Eun-Kyung Park | - |
dc.contributor.googleauthor | Se-Hoon Kim | - |
dc.contributor.googleauthor | Jong Hee Chang | - |
dc.contributor.googleauthor | Dong-Seok Kim | - |
dc.contributor.googleauthor | Yong-Kil Hong | - |
dc.contributor.googleauthor | Sun Ho Kim | - |
dc.contributor.googleauthor | Seok-Gu Kang | - |
dc.contributor.googleauthor | Frederick F. Lang | - |
dc.identifier.doi | 10.1007/s00381-012-1963-x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00036 | - |
dc.contributor.localId | A00402 | - |
dc.contributor.localId | A00560 | - |
dc.contributor.localId | A00610 | - |
dc.contributor.localId | A00837 | - |
dc.contributor.localId | A01607 | - |
dc.contributor.localId | A03470 | - |
dc.contributor.localId | A04359 | - |
dc.contributor.localId | A03218 | - |
dc.relation.journalcode | J00525 | - |
dc.identifier.eissn | 1433-0350 | - |
dc.identifier.pmid | 23143001 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs00381-012-1963-x | - |
dc.subject.keyword | Glioma | - |
dc.subject.keyword | Glioma cancer stem cell | - |
dc.subject.keyword | Stem cell niche | - |
dc.subject.keyword | Serial subtransplantation | - |
dc.contributor.alternativeName | Kang, Seok Gu | - |
dc.contributor.alternativeName | Kim, Dong Seok | - |
dc.contributor.alternativeName | Kim, Sun Ho | - |
dc.contributor.alternativeName | Kim, Se Hoon | - |
dc.contributor.alternativeName | Kim, Eui Hyun | - |
dc.contributor.alternativeName | Park, Eun Kyung | - |
dc.contributor.alternativeName | Lee, Ji Hyun | - |
dc.contributor.alternativeName | Chang, Jong Hee | - |
dc.contributor.alternativeName | Huh, Yong Min | - |
dc.contributor.affiliatedAuthor | Kang, Seok Gu | - |
dc.contributor.affiliatedAuthor | Kim, Dong Seok | - |
dc.contributor.affiliatedAuthor | Kim, Sun Ho | - |
dc.contributor.affiliatedAuthor | Kim, Se Hoon | - |
dc.contributor.affiliatedAuthor | Kim, Eui Hyun | - |
dc.contributor.affiliatedAuthor | Park, Eun Kyung | - |
dc.contributor.affiliatedAuthor | Chang, Jong Hee | - |
dc.contributor.affiliatedAuthor | Huh, Yong Min | - |
dc.contributor.affiliatedAuthor | Lee, Ji Hyun | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 29 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 55 | - |
dc.citation.endPage | 64 | - |
dc.identifier.bibliographicCitation | CHILDS NERVOUS SYSTEM, Vol.29(1) : 55-64, 2013 | - |
dc.identifier.rimsid | 28829 | - |
dc.type.rims | ART | - |
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