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Biological Function and Potential Applications of Garcinol in Human Dental Pulp Stem Cells

 Sunmi Jang  ;  Uk-Seong Kim  ;  Sukjoon Lee  ;  Euiseong Kim  ;  Han-Sung Jung  ;  Su-Jung Shin  ;  Sumi Kang  ;  Insoon Chang  ;  Sunil Kim 
 JOURNAL OF ENDODONTICS, Vol.49(12) : 1652-1659, 2023-12 
Journal Title
Issue Date
Animals ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Dental Pulp* ; Humans ; Mice ; Osteogenesis* ; Stem Cells / physiology
Gene expression ; odontogenesis ; osteogenesis ; pulp biology ; single cell sequencing ; stem cell
Introduction: The regeneration of pulp tissue is crucial for true regenerative endodontic treatment, which requires a reduction in osteogenic differentiation. Garcinol, a histone acetyltransferase inhibitor, is a natural regulator that is known to suppress the osteogenic differentiation of dental pulp stem cells. In this study, the inhibitory effect of garcinol on the osteogenic differentiation of human dental pulp stem cells (hDPSCs) was evaluated using three-dimensional culture under in vitro and in vivo conditions. Methods: hDPSCs were obtained from caries-free third molars and cultured with 10 mM garcinol for 7 days in an ultra -low attachment plate. The cell stemness and expression of osteogenic differentiation-related genes were analyzed using reverse transcription-polymerase chain reaction and single-cell analysis. A transplantation experiment was performed in mice to investigate whether garcinol-treated hDPSCs showed restrained osteogenic differentiation. Results: hDPSCs cultured in the U-shaped ultra-low attachment plate showed the highest expression of stemness-related genes. Garcinol-treated hDPSCs demonstrated downregulation of osteogenic differentiation, with lower expression of bone sialoprotein, which is related to bone formation, and higher expression of dentin sialophosphoprotein, which is related to dentin formation. However, the garcinol-treated hDPSCs did not show any alterations in their stemness. Consistent results were observed in the transplantation experiment in mice. Conclusions: Garcinol reduced the osteogenic differentiation of hDPSCs, which can contribute to true regenerative endodontic treatment. (J Endod 2023;49:1652-1659.)
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2. College of Dentistry (치과대학) > Dept. of Dental Education (치의학교육학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kang, Sumi(강수미) ORCID logo https://orcid.org/0000-0002-4682-6540
Kim, Sun Il(김선일) ORCID logo https://orcid.org/0000-0002-8889-9844
Kim, Eui Seong(김의성) ORCID logo https://orcid.org/0000-0003-2126-4761
Shin, Su Jung(신수정) ORCID logo https://orcid.org/0000-0001-5795-3919
Lee, Sukjoon(이석준)
Jang, Sun Mi(장선미)
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
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