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Multistability and hysteresis in states of oral microbiota: Is it impacting the dental clinical cohort studies?

Authors
 Utkarsh Mangal  ;  Kowoon Noh  ;  Seeyoon Lee  ;  Jae-Kook Cha  ;  Je Seon Song  ;  Jung-Yul Cha  ;  Kee-Joon Lee  ;  Kwang-Mahn Kim  ;  Jae-Sung Kwon  ;  Sung-Hwan Choi 
Citation
 JOURNAL OF PERIODONTAL RESEARCH, Vol.58(2) : 381-391, 2023-04 
Journal Title
JOURNAL OF PERIODONTAL RESEARCH
ISSN
 0022-3484 
Issue Date
2023-04
MeSH
Biomarkers ; Cohort Studies ; Humans ; Microbiota* / genetics ; Micrococcaceae* / genetics ; RNA, Ribosomal, 16S / genetics
Keywords
16S rRNA gene sequencing ; dysbiosis ; microbiomes ; multistability ; temporal variability
Abstract
Introduction: Microbiome from a "healthy cohort" is used as a reference for comparison to cases and intervention. However, the studies with cohort-based clinical research have not sufficiently accounted for the multistability in oral microbial community. The screening is limited to phenotypic features with marked variations in microbial genomic markers. Herein, we aimed to assess the stability of the oral microbiome across time from an intervention-free "healthy" cohort.

Methods: We obtained 33 supragingival samples of 11 healthy participants from the biobank. For each participant, we processed one sample as baseline (T0) and two samples spaced at 1-month (T1) and 3-month (T2) intervals for 16S ribosomal RNA gene sequencing analysis.

Results: We observed that taxonomic profiling had a similar pattern of dominant genera, namely, Rothia, Prevotella, and Hemophilus, at all time points. Shannon diversity revealed a significant increase from T0 (p < .05). Bray Curtis dissimilarity was significant (R = -.02, p < .01) within the cohort at each time point. Community stability had negative correlation to synchrony (r = -.739; p = .009) and variance (r = -.605; p = .048) of the species. Clustering revealed marked differences in the grouping patterns between the three time points. For all time points, the clusters presented a substantially dissimilar set of differentially abundant taxonomic and functional biomarkers.

Conclusion: Our observations indicate towards the presence of multistable states within the oral microbiome in an intervention-free healthy cohort. For a conclusive and meaningful long-term reference, dental clinical research should account for multistability in the personalized therapy approach to improve the identification and classification of reliable markers.
Full Text
https://onlinelibrary.wiley.com/doi/10.1111/jre.13098
DOI
10.1111/jre.13098
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Orthodontics (교정과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Jae-Sung(권재성) ORCID logo https://orcid.org/0000-0001-9803-7730
Kim, Kwang Mahn(김광만) ORCID logo https://orcid.org/0000-0002-5235-0294
Mangal, Utkarsh(만갈웃커시)
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Lee, Kee Joon(이기준) ORCID logo https://orcid.org/0000-0002-0782-3128
Cha, Jae Kook(차재국) ORCID logo https://orcid.org/0000-0001-6817-9834
Cha, Jung Yul(차정열)
Choi, Sung Hwan(최성환) ORCID logo https://orcid.org/0000-0002-1150-0268
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/195343
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