13 55

Cited 0 times in

Temporal Transcriptome Analysis of SARS-CoV-2-Infected Lung and Spleen in Human ACE2-Transgenic Mice

 Jung Ah Kim  ;  Sung-Hee Kim  ;  Jung Seon Seo  ;  Hyuna Noh  ;  Haengdueng Jeong  ;  Jiseon Kim  ;  Donghun Jeon  ;  Jeong Jin Kim  ;  Dain On  ;  Suhyeon Yoon  ;  Sang Gyu Lee  ;  Youn Woo Lee  ;  Hui Jeong Jang  ;  In Ho Park  ;  Jooyeon Oh  ;  Sang-Hyuk Seok  ;  Yu Jin Lee  ;  Seung-Min Hong  ;  Se-Hee An  ;  Joon-Yong Bae  ;  Jung-Ah Choi  ;  Seo Yeon Kim  ;  Young Been Kim  ;  Ji-Yeon Hwang  ;  Hyo-Jung Lee  ;  Hong Bin Kim  ;  Dae Gwin Jeong  ;  Daesub Song  ;  Manki Song  ;  Man-Seong Park  ;  Kang-Seuk Choi  ;  Jun Won Park  ;  Jun-Won Yun  ;  Jeon-Soo Shin  ;  Ho-Young Lee  ;  Jun-Young Seo  ;  Ki Taek Nam  ;  Heon Yung Gee  ;  Je Kyung Seong 
 MOLECULES AND CELLS, Vol.45(12) : 896-910, 2022-12 
Journal Title
Issue Date
Angiotensin-Converting Enzyme 2 / genetics ; Animals ; COVID-19* / genetics ; Cytokines ; Disease Models, Animal ; Gene Expression Profiling ; Humans ; Lung ; Mice ; Mice, Transgenic ; SARS-CoV-2 ; Spleen / metabolism ; Transcriptome ; Virus Diseases*
SARS-CoV-2 ; immune-mediated response ; transcriptome profiling
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly transmissible and potentially fatal virus. So far, most comprehensive analyses encompassing clinical and transcriptional manifestation have concentrated on the lungs. Here, we confirmed evident signs of viral infection in the lungs and spleen of SARS-CoV-2-infected K18-hACE2 mice, which replicate the phenotype and infection symptoms in hospitalized humans. Seven days post viral detection in organs, infected mice showed decreased vital signs, leading to death. Bronchopneumonia due to infiltration of leukocytes in the lungs and reduction in the spleen lymphocyte region were observed. Transcriptome profiling implicated the meticulous regulation of distress and recovery from cytokine-mediated immunity by distinct immune cell types in a time-dependent manner. In lungs, the chemokine-driven response to viral invasion was highly elevated at 2 days post infection (dpi). In late infection, diseased lungs, post the innate immune process, showed recovery signs. The spleen established an even more immediate line of defense than the lungs, and the cytokine expression profile dropped at 7 dpi. At 5 dpi, spleen samples diverged into two distinct groups with different transcriptome profile and pathophysiology. Inhibition of consecutive host cell viral entry and massive immunoglobulin production and proteolysis inhibition seemed that one group endeavored to survive, while the other group struggled with developmental regeneration against consistent viral intrusion through the replication cycle. Our results may contribute to improved understanding of the longitudinal response to viral infection and development of potential therapeutics for hospitalized patients affected by SARS-CoV-2.
Files in This Item:
T202300099.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sung-Hee(김성희)
Nam, Ki Taek(남기택)
Park, Inho(박인호) ORCID logo https://orcid.org/0000-0003-2190-5469
Seo, Jun Young(서준영) ORCID logo https://orcid.org/0000-0003-4004-2013
Shin, Jeon Soo(신전수) ORCID logo https://orcid.org/0000-0002-8294-3234
Gee, Heon Yung(지헌영) ORCID logo https://orcid.org/0000-0002-8741-6177
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.