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Temporal Transcriptome Analysis of SARS-CoV-2-Infected Lung and Spleen in Human ACE2-Transgenic Mice

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dc.contributor.author김성희-
dc.contributor.author남기택-
dc.contributor.author박인호-
dc.contributor.author서준영-
dc.contributor.author신전수-
dc.contributor.author지헌영-
dc.date.accessioned2023-03-03T02:49:30Z-
dc.date.available2023-03-03T02:49:30Z-
dc.date.issued2022-12-
dc.identifier.issn1016-8478-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/192906-
dc.description.abstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly transmissible and potentially fatal virus. So far, most comprehensive analyses encompassing clinical and transcriptional manifestation have concentrated on the lungs. Here, we confirmed evident signs of viral infection in the lungs and spleen of SARS-CoV-2-infected K18-hACE2 mice, which replicate the phenotype and infection symptoms in hospitalized humans. Seven days post viral detection in organs, infected mice showed decreased vital signs, leading to death. Bronchopneumonia due to infiltration of leukocytes in the lungs and reduction in the spleen lymphocyte region were observed. Transcriptome profiling implicated the meticulous regulation of distress and recovery from cytokine-mediated immunity by distinct immune cell types in a time-dependent manner. In lungs, the chemokine-driven response to viral invasion was highly elevated at 2 days post infection (dpi). In late infection, diseased lungs, post the innate immune process, showed recovery signs. The spleen established an even more immediate line of defense than the lungs, and the cytokine expression profile dropped at 7 dpi. At 5 dpi, spleen samples diverged into two distinct groups with different transcriptome profile and pathophysiology. Inhibition of consecutive host cell viral entry and massive immunoglobulin production and proteolysis inhibition seemed that one group endeavored to survive, while the other group struggled with developmental regeneration against consistent viral intrusion through the replication cycle. Our results may contribute to improved understanding of the longitudinal response to viral infection and development of potential therapeutics for hospitalized patients affected by SARS-CoV-2.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherKorean Society for Molecular and Cellular Biology-
dc.relation.isPartOfMOLECULES AND CELLS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAngiotensin-Converting Enzyme 2 / genetics-
dc.subject.MESHAnimals-
dc.subject.MESHCOVID-19* / genetics-
dc.subject.MESHCytokines-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHGene Expression Profiling-
dc.subject.MESHHumans-
dc.subject.MESHLung-
dc.subject.MESHMice-
dc.subject.MESHMice, Transgenic-
dc.subject.MESHSARS-CoV-2-
dc.subject.MESHSpleen / metabolism-
dc.subject.MESHTranscriptome-
dc.subject.MESHVirus Diseases*-
dc.titleTemporal Transcriptome Analysis of SARS-CoV-2-Infected Lung and Spleen in Human ACE2-Transgenic Mice-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentBioMedical Science Institute (의생명과학부)-
dc.contributor.googleauthorJung Ah Kim-
dc.contributor.googleauthorSung-Hee Kim-
dc.contributor.googleauthorJung Seon Seo-
dc.contributor.googleauthorHyuna Noh-
dc.contributor.googleauthorHaengdueng Jeong-
dc.contributor.googleauthorJiseon Kim-
dc.contributor.googleauthorDonghun Jeon-
dc.contributor.googleauthorJeong Jin Kim-
dc.contributor.googleauthorDain On-
dc.contributor.googleauthorSuhyeon Yoon-
dc.contributor.googleauthorSang Gyu Lee-
dc.contributor.googleauthorYoun Woo Lee-
dc.contributor.googleauthorHui Jeong Jang-
dc.contributor.googleauthorIn Ho Park-
dc.contributor.googleauthorJooyeon Oh-
dc.contributor.googleauthorSang-Hyuk Seok-
dc.contributor.googleauthorYu Jin Lee-
dc.contributor.googleauthorSeung-Min Hong-
dc.contributor.googleauthorSe-Hee An-
dc.contributor.googleauthorJoon-Yong Bae-
dc.contributor.googleauthorJung-Ah Choi-
dc.contributor.googleauthorSeo Yeon Kim-
dc.contributor.googleauthorYoung Been Kim-
dc.contributor.googleauthorJi-Yeon Hwang-
dc.contributor.googleauthorHyo-Jung Lee-
dc.contributor.googleauthorHong Bin Kim-
dc.contributor.googleauthorDae Gwin Jeong-
dc.contributor.googleauthorDaesub Song-
dc.contributor.googleauthorManki Song-
dc.contributor.googleauthorMan-Seong Park-
dc.contributor.googleauthorKang-Seuk Choi-
dc.contributor.googleauthorJun Won Park-
dc.contributor.googleauthorJun-Won Yun-
dc.contributor.googleauthorJeon-Soo Shin-
dc.contributor.googleauthorHo-Young Lee-
dc.contributor.googleauthorJun-Young Seo-
dc.contributor.googleauthorKi Taek Nam-
dc.contributor.googleauthorHeon Yung Gee-
dc.contributor.googleauthorJe Kyung Seong-
dc.identifier.doi10.14348/molcells.2022.0089-
dc.contributor.localIdA06017-
dc.contributor.localIdA01243-
dc.contributor.localIdA01631-
dc.contributor.localIdA01911-
dc.contributor.localIdA02144-
dc.contributor.localIdA03971-
dc.relation.journalcodeJ02273-
dc.identifier.eissn0219-1032-
dc.identifier.pmid36324270-
dc.subject.keywordSARS-CoV-2-
dc.subject.keywordimmune-mediated response-
dc.subject.keywordtranscriptome profiling-
dc.contributor.alternativeNameKim, Sung-Hee-
dc.contributor.affiliatedAuthor김성희-
dc.contributor.affiliatedAuthor남기택-
dc.contributor.affiliatedAuthor박인호-
dc.contributor.affiliatedAuthor서준영-
dc.contributor.affiliatedAuthor신전수-
dc.contributor.affiliatedAuthor지헌영-
dc.citation.volume45-
dc.citation.number12-
dc.citation.startPage896-
dc.citation.endPage910-
dc.identifier.bibliographicCitationMOLECULES AND CELLS, Vol.45(12) : 896-910, 2022-12-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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