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The collagen structure of C1q induces wound healing by engaging discoidin domain receptor 2

Authors
 Ria Aryani Hayuningtyas  ;  Myeonggil Han  ;  Seoyeon Choi  ;  Man Sup Kwak  ;  In Ho Park  ;  Ji-Hyun Lee  ;  Ji Eun Choi  ;  Dae Ki Kim  ;  Myoungsun Son  ;  Jeon-Soo Shin 
Citation
 MOLECULAR MEDICINE, Vol.27(1) : 125, 2021-10 
Journal Title
MOLECULAR MEDICINE
ISSN
 1076-1551 
Issue Date
2021-10
Keywords
C1q ; Collagen ; DDR2 ; Wound healing
Abstract
Background: C1q has been reported to reveal complement-independent roles in immune and non-immune cells. C1q binds to its specific receptors to regulate distinct functions that rely on the environment and cell types. Discoidin domain receptor 2 (DDR2) is activated by collagen and functions in wound healing by controlling matrix metalloproteinase (MMP) expression. Since C1q exhibits a collagen-like structure, we hypothesized that C1q might engage DDR2 to regulate wound healing and extracellular matrix (ECM) remodeling.

Methods: Cell-based assay, proximity ligation assay, ELISA, and surface plasmon analysis were utilized to investigate DDR2 and C1q binding. We also investigate the C1q-mediated in vitro wound healing ability using the human fibrosarcoma cell line, HT1080.

Results: C1q induced the phosphorylation of DDR2, p38 kinase, and ERK1/2. C1q and DDR2 binding improved cell migration and induced MMP2 and MMP9 expression. DDR2-specific shRNA reduced C1q-mediated cell migration for wound healing.

Conclusions: C1q is a new DDR2 ligand that promotes wound healing. These findings have therapeutic implications in wound healing-related diseases.
Files in This Item:
T202104352.pdf Download
DOI
10.1186/s10020-021-00388-y
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kwak, Man Sup(곽만섭) ORCID logo https://orcid.org/0000-0002-3989-3016
Park, Inho(박인호) ORCID logo https://orcid.org/0000-0003-2190-5469
Shin, Jeon Soo(신전수) ORCID logo https://orcid.org/0000-0002-8294-3234
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/185936
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