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Monoclonal antibodies with various reactivity to p58 killer inhibitory receptors

Authors
 JEON-SOO SHIN  ;  EUI-CHEOL SHIN  ;  JONGSUN KIM  ;  IN-HONG CHOI  ;  JEON-HAN PARK  ;  SE-JONG KIM 
Citation
 Hybridoma, Vol.18(6) : 521-527, 1999 
Journal Title
 Hybridoma 
ISSN
 0272-457X 
Issue Date
1999
MeSH
Animals ; Antibodies, Monoclonal/immunology* ; Antibodies, Monoclonal/metabolism ; Antibody Specificity ; Blotting, Western ; CD4 Antigens/analysis ; CD56 Antigen/analysis ; CD8 Antigens/analysis ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; Epitopes ; Hybridomas/immunology ; Killer Cells, Natural/immunology* ; Killer Cells, Natural/metabolism ; Leukocytes, Mononuclear/immunology ; Mice ; Mice, Inbred BALB C ; Receptors, IgG/analysis ; Receptors, Immunologic/genetics ; Receptors, Immunologic/immunology* ; Receptors, Immunologic/metabolism ; Receptors, KIR ; Receptors, KIR2DL1 ; Receptors, KIR2DL3 ; Receptors, Natural Killer Cell ; Recombinant Proteins/immunology ; Recombinant Proteins/metabolism ; T-Lymphocyte Subsets/immunology
Abstract
Human natural killer (NK) cells have receptors that bind to HLA class I molecules. Among these receptors, p58 and p50 bind to HLA-C. P58 belongs to the killer inhibitory receptor (KIR) and p50 belongs to the killer activatory receptor (KAR). Previously, we obtained three recombinant p58 KIR or p50 KAR proteins, KAR-K1 (KIR2DS4), KIR-K6 (KIR2DL1), and KIR-K7 (KIR2DL3). In this study, we produced and characterized seven monoclonal antibodies (MAbs) to the p58 KIR and p50 KAR proteins. The MAbs were classified in three groups according to their antigen-binding specificity: 5IB103 and 26ID707 were KAR-K1-specific; A809, 190IIC311, and 197IIC611 were KIR-K7-specific; while A210 and A803g bound to all three recombinant proteins. The MAbs reactive to KIR-K7 bound to the gamma3 domain among two immunoglobulin (Ig) domains of KIR-K7. Immunofluorescence staining and flow cytometric analysis with A803g showed reactivity to about 10% of peripheral blood mononuclear cells and 35% of purified natural killer cells. Double immunofluorescence staining with A803g and anti-CD56 Ab showed that CD56 and p58 or p50 were expressed on NK cells in a mutually exclusive way. We also investigated T-cell markers in A803g+ cells. A803g+ T cells were almost CD8+ cells. MAbs produced in this study can be utilized practically in the investigation of biological characteristics of p58 KIR and p50 KAR.
Full Text
https://www.liebertpub.com/doi/abs/10.1089/hyb.1999.18.521
DOI
10.1089/hyb.1999.18.521
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Jong(김세종)
Kim, Jong Sun(김종선) ORCID logo https://orcid.org/0000-0002-3149-669X
Park, Jeon Han(박전한) ORCID logo https://orcid.org/0000-0001-9604-3205
Shin, Jeon Soo(신전수) ORCID logo https://orcid.org/0000-0002-8294-3234
Choi, In Hong(최인홍) ORCID logo https://orcid.org/0000-0001-9851-0137
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174162
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