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Monoclonal antibodies with various reactivity to p58 killer inhibitory receptors
DC Field | Value | Language |
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dc.contributor.author | 김세종 | - |
dc.contributor.author | 김종선 | - |
dc.contributor.author | 박전한 | - |
dc.contributor.author | 신전수 | - |
dc.contributor.author | 최인홍 | - |
dc.date.accessioned | 2020-01-23T05:17:45Z | - |
dc.date.available | 2020-01-23T05:17:45Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 0272-457X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/174162 | - |
dc.description.abstract | Human natural killer (NK) cells have receptors that bind to HLA class I molecules. Among these receptors, p58 and p50 bind to HLA-C. P58 belongs to the killer inhibitory receptor (KIR) and p50 belongs to the killer activatory receptor (KAR). Previously, we obtained three recombinant p58 KIR or p50 KAR proteins, KAR-K1 (KIR2DS4), KIR-K6 (KIR2DL1), and KIR-K7 (KIR2DL3). In this study, we produced and characterized seven monoclonal antibodies (MAbs) to the p58 KIR and p50 KAR proteins. The MAbs were classified in three groups according to their antigen-binding specificity: 5IB103 and 26ID707 were KAR-K1-specific; A809, 190IIC311, and 197IIC611 were KIR-K7-specific; while A210 and A803g bound to all three recombinant proteins. The MAbs reactive to KIR-K7 bound to the gamma3 domain among two immunoglobulin (Ig) domains of KIR-K7. Immunofluorescence staining and flow cytometric analysis with A803g showed reactivity to about 10% of peripheral blood mononuclear cells and 35% of purified natural killer cells. Double immunofluorescence staining with A803g and anti-CD56 Ab showed that CD56 and p58 or p50 were expressed on NK cells in a mutually exclusive way. We also investigated T-cell markers in A803g+ cells. A803g+ T cells were almost CD8+ cells. MAbs produced in this study can be utilized practically in the investigation of biological characteristics of p58 KIR and p50 KAR. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Mary Ann Liebert | - |
dc.relation.isPartOf | Hybridoma | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antibodies, Monoclonal/immunology* | - |
dc.subject.MESH | Antibodies, Monoclonal/metabolism | - |
dc.subject.MESH | Antibody Specificity | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | CD4 Antigens/analysis | - |
dc.subject.MESH | CD56 Antigen/analysis | - |
dc.subject.MESH | CD8 Antigens/analysis | - |
dc.subject.MESH | Electrophoresis, Polyacrylamide Gel | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay | - |
dc.subject.MESH | Epitopes | - |
dc.subject.MESH | Hybridomas/immunology | - |
dc.subject.MESH | Killer Cells, Natural/immunology* | - |
dc.subject.MESH | Killer Cells, Natural/metabolism | - |
dc.subject.MESH | Leukocytes, Mononuclear/immunology | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred BALB C | - |
dc.subject.MESH | Receptors, IgG/analysis | - |
dc.subject.MESH | Receptors, Immunologic/genetics | - |
dc.subject.MESH | Receptors, Immunologic/immunology* | - |
dc.subject.MESH | Receptors, Immunologic/metabolism | - |
dc.subject.MESH | Receptors, KIR | - |
dc.subject.MESH | Receptors, KIR2DL1 | - |
dc.subject.MESH | Receptors, KIR2DL3 | - |
dc.subject.MESH | Receptors, Natural Killer Cell | - |
dc.subject.MESH | Recombinant Proteins/immunology | - |
dc.subject.MESH | Recombinant Proteins/metabolism | - |
dc.subject.MESH | T-Lymphocyte Subsets/immunology | - |
dc.title | Monoclonal antibodies with various reactivity to p58 killer inhibitory receptors | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.googleauthor | JEON-SOO SHIN | - |
dc.contributor.googleauthor | EUI-CHEOL SHIN | - |
dc.contributor.googleauthor | JONGSUN KIM | - |
dc.contributor.googleauthor | IN-HONG CHOI | - |
dc.contributor.googleauthor | JEON-HAN PARK | - |
dc.contributor.googleauthor | SE-JONG KIM | - |
dc.identifier.doi | 10.1089/hyb.1999.18.521 | - |
dc.contributor.localId | A00603 | - |
dc.contributor.localId | A00921 | - |
dc.contributor.localId | A01641 | - |
dc.contributor.localId | A02144 | - |
dc.contributor.localId | A04167 | - |
dc.relation.journalcode | J03747 | - |
dc.identifier.pmid | 10626681 | - |
dc.identifier.url | https://www.liebertpub.com/doi/abs/10.1089/hyb.1999.18.521 | - |
dc.contributor.alternativeName | Kim, Se Jong | - |
dc.contributor.affiliatedAuthor | 김세종 | - |
dc.contributor.affiliatedAuthor | 김종선 | - |
dc.contributor.affiliatedAuthor | 박전한 | - |
dc.contributor.affiliatedAuthor | 신전수 | - |
dc.contributor.affiliatedAuthor | 최인홍 | - |
dc.citation.volume | 18 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 521 | - |
dc.citation.endPage | 527 | - |
dc.identifier.bibliographicCitation | Hybridoma, Vol.18(6) : 521-527, 1999 | - |
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