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Microsphere-Based Nanoindentation for the Monitoring of Cellular Cortical Stiffness Regulated by MT1-MMP

Authors
 Minhee Ku  ;  Hyun‐Joon Kim  ;  Su Yee Yau  ;  Nara Yoon  ;  Nam Hee Kim  ;  Jong In Yook  ;  Jin‐Suck Suh  ;  Dae‐Eun Kim  ;  Jaemoon Yang 
Citation
 Small, Vol.14(41) : e1803000, 2018 
Journal Title
 Small 
ISSN
 1613-6810 
Issue Date
2018
Keywords
MT1‐MMP ; atomic force microscopy ; cancer mechanobiology ; cortical stiffness ; nanoindentation
Abstract
Biophysical properties are intimately connected to metastatic functions and aggressiveness in cancers. Especially, cellular stiffness is regarded as a biomarker for the understanding of metastatic potential and drug sensitivity. Here, protease-mediated changes of cortical stiffness are identified due to the deformation of cytoskeleton alignment at a cortex. For the past few decades, membrane type 1-matrix metalloproteinase (MT1-MMP) has been well known as a kernel protease enriched in podosomes during metastasis for extracellular matrix degradation. However, the biophysical significance of MT1-MMP expressing cancer cells is still unknown. Therefore, the nanomechanics of cancer cells is analyzed by a nanoindentation using a microsphere-attached cantilever of atomic force microscopy (AFM). In conclusion, the results suggest that MT1-MMP has contributed as a key regulator in cytoskeletal deformation related with cancer metastasis. Particularly, the AFM-based nanoindentation system for the monitoring of cortical nanomechanics will be crucial to understand molecular networks in cancers.
Full Text
https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.201803000
DOI
10.1002/smll.201803000
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
Ku, Min Hee(구민희) ORCID logo https://orcid.org/0000-0002-1674-1474
Kim, Nam Hee(김남희) ORCID logo https://orcid.org/0000-0002-3087-5276
Suh, Jin Suck(서진석) ORCID logo https://orcid.org/0000-0001-9455-9240
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
Yook, Jong In(육종인) ORCID logo https://orcid.org/0000-0002-7318-6112
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/164985
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