The purpose of this study was to develop a nursing education program using a web-based learning system to teach peritoneal dialysis and to evaluate the effectiveness of the program. The program was developed in two steps. The first step was to develop the content using CVI, and the second was to program it using HTML. The program which was developed was evaluated with 50 nurses of whom 48 responded to the questionnaire. The results are as follows: Of the subjects, 85.4% responded that they were satisfied with this web-based learning system and 87.5% stated that they would recommend this system to nurses who are in other nursing education programs. Of the educational material characteristics, the item that the subjects evaluated as most valuable was "accuracy of the content". For system related characteristics, the item to which the subjects gave the most positive response was "clarity of the system tool marking" (81.3%). In conclusion, it was verified that a web-based learning system is an effective and satisfactory method for nursing education.