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The full repertoire of Drosophila gustatory receptors for detecting an aversive compound

Authors
 Jaewon Shim  ;  Youngseok Lee  ;  Yong Taek Jeong  ;  Yonjung Kim  ;  Min Goo Lee  ;  Craig Montell  ;  Seok Jun Moon 
Citation
 Nature Communications, Vol.6 : 8867, 2015 
Journal Title
 Nature Communications 
Issue Date
2015
MeSH
Animals ; Avoidance Learning* ; Canavanine/metabolism* ; Chemoreceptor Cells/metabolism ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism* ; Drosophila melanogaster ; Insecticides/metabolism* ; Patch-Clamp Techniques ; Receptors, Cell Surface/genetics ; Receptors, Cell Surface/metabolism* ; Taste Buds/metabolism*
Abstract
The ability to detect toxic compounds in foods is essential for animal survival. However, the minimal subunit composition of gustatory receptors required for sensing aversive chemicals in Drosophila is unknown. Here we report that three gustatory receptors, GR8a, GR66a and GR98b function together in the detection of L-canavanine, a plant-derived insecticide. Ectopic co-expression of Gr8a and Gr98b in Gr66a-expressing, bitter-sensing gustatory receptor neurons (GRNs) confers responsiveness to L-canavanine. Furthermore, misexpression of all three Grs enables salt- or sweet-sensing GRNs to respond to L-canavanine. Introduction of these Grs in sweet-sensing GRNs switches L-canavanine from an aversive to an attractive compound. Co-expression of GR8a, GR66a and GR98b in Drosophila S2 cells induces an L-canavanine-activated nonselective cation conductance. We conclude that three GRs collaborate to produce a functional L-canavanine receptor. Thus, our results clarify the full set of GRs underlying the detection of a toxic tastant that drives avoidance behaviour in an insect.
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DOI
10.1038/ncomms9867
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실)
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실)
Yonsei Authors
김연정(Kim, Yon Jung) ORCID logo https://orcid.org/0000-0003-0251-8711
문석준(Moon, Seok Jun) ORCID logo https://orcid.org/0000-0001-7282-2888
이민구(Lee, Min Goo) ORCID logo https://orcid.org/0000-0001-7436-012X
정용택(Jeong, Yong Taek)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/156745
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