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Quantitative detection of telomerase activity by real-time TRAP assay in the body fluids of cancer patients

Authors
 Woo Young Shim  ;  Kyu Hyun Park  ;  Hei-Chul Jeung  ;  Yong Tae Kim  ;  Tae Soo Kim  ;  Woo Jin Hyung  ;  Sung Hwan An  ;  Sang Hwa Yang  ;  Sung Hoon Noh  ;  Hyun Cheol Chung  ;  Sun Young Rha 
Citation
 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, Vol.16(5) : 857-863, 2005 
Journal Title
 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 
ISSN
 1107-3756 
Issue Date
2005
MeSH
Body Fluids/chemistry* ; Humans ; Neoplasms/diagnosis* ; Neoplasms/enzymology ; Polymerase Chain Reaction/methods* ; Sensitivity and Specificity ; Telomerase/analysis* ; Telomerase/genetics ; Telomerase/metabolism ; Tumor Cells, Cultured
Keywords
16211255
Abstract
Real-time TRAP assay was developed to improve the sensitivity and quantitative detection of telomerase activity in the body fluids of cancer patients. The sensitivity and clinical significance of the real-time TRAP assay was investigated. Real-time PCR protocol was modified by using ACX primer and SYBR green mixture from the process of TS primer extension. Real-time TRAP showed high correlation (r2=0.843, p=0.001) and sensitivity (25 times higher) compared to conventional TRAP. Of 164 samples, there were 8 positives in cytology (4.9%), 7 (4.3%) using the conventional TRAP, and 41 (25%) using real-time TRAP. In cytology positive samples, real-time TRAP showed a higher positivity than conventional TRAP (75% vs 63%) suggesting a higher sensitivity in the body fluids. There was a tendency towards a longer progression-free duration in telomerase negative patients than in positive patients, as determined by conventional and real-time TRAP. The diagnostic interval between the first positivity documentation and clinical progression was short in the order of real-time TRAP, conventional TRAP and cytology. In conclusion, real-time TRAP assay can detect telomerase activity at an earlier phase of cancer progression and can replace conventional TRAP assay for detecting the telomerase activity in body fluids.
Full Text
http://www.spandidos-publications.com/ijmm/16/5/857
DOI
OAK-2005-04832
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Cancer Metastasis Research Center (암전이연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Noh, Sung Hoon(노성훈) ORCID logo https://orcid.org/0000-0003-4386-6886
Rha, Sun Young(라선영) ORCID logo https://orcid.org/0000-0002-2512-4531
Yang, Sang Hwa(양상화)
Chung, Hyun Cheol(정현철) ORCID logo https://orcid.org/0000-0002-0920-9471
Jeung, Hei Cheul(정희철) ORCID logo https://orcid.org/0000-0003-0952-3679
Hyung, Woo Jin(형우진) ORCID logo https://orcid.org/0000-0002-8593-9214
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/150753
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