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Quantitative detection of telomerase activity by real-time TRAP assay in the body fluids of cancer patients
DC Field | Value | Language |
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dc.contributor.author | 노성훈 | - |
dc.contributor.author | 라선영 | - |
dc.contributor.author | 양상화 | - |
dc.contributor.author | 정현철 | - |
dc.contributor.author | 정희철 | - |
dc.contributor.author | 형우진 | - |
dc.date.accessioned | 2017-10-26T06:17:25Z | - |
dc.date.available | 2017-10-26T06:17:25Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1107-3756 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/150753 | - |
dc.description.abstract | Real-time TRAP assay was developed to improve the sensitivity and quantitative detection of telomerase activity in the body fluids of cancer patients. The sensitivity and clinical significance of the real-time TRAP assay was investigated. Real-time PCR protocol was modified by using ACX primer and SYBR green mixture from the process of TS primer extension. Real-time TRAP showed high correlation (r2=0.843, p=0.001) and sensitivity (25 times higher) compared to conventional TRAP. Of 164 samples, there were 8 positives in cytology (4.9%), 7 (4.3%) using the conventional TRAP, and 41 (25%) using real-time TRAP. In cytology positive samples, real-time TRAP showed a higher positivity than conventional TRAP (75% vs 63%) suggesting a higher sensitivity in the body fluids. There was a tendency towards a longer progression-free duration in telomerase negative patients than in positive patients, as determined by conventional and real-time TRAP. The diagnostic interval between the first positivity documentation and clinical progression was short in the order of real-time TRAP, conventional TRAP and cytology. In conclusion, real-time TRAP assay can detect telomerase activity at an earlier phase of cancer progression and can replace conventional TRAP assay for detecting the telomerase activity in body fluids. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | D.A. Spandidos | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Body Fluids/chemistry* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Neoplasms/diagnosis* | - |
dc.subject.MESH | Neoplasms/enzymology | - |
dc.subject.MESH | Polymerase Chain Reaction/methods* | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | Telomerase/analysis* | - |
dc.subject.MESH | Telomerase/genetics | - |
dc.subject.MESH | Telomerase/metabolism | - |
dc.subject.MESH | Tumor Cells, Cultured | - |
dc.title | Quantitative detection of telomerase activity by real-time TRAP assay in the body fluids of cancer patients | - |
dc.type | Article | - |
dc.publisher.location | Greece | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | Research Institutes (연구소) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학교실) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.department | Cancer Metastasis Research Center (암전이연구센터) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.department | Dept. of Surgery (외과학교실) | - |
dc.contributor.googleauthor | Woo Young Shim | - |
dc.contributor.googleauthor | Kyu Hyun Park | - |
dc.contributor.googleauthor | Hei-Chul Jeung | - |
dc.contributor.googleauthor | Yong Tae Kim | - |
dc.contributor.googleauthor | Tae Soo Kim | - |
dc.contributor.googleauthor | Woo Jin Hyung | - |
dc.contributor.googleauthor | Sung Hwan An | - |
dc.contributor.googleauthor | Sang Hwa Yang | - |
dc.contributor.googleauthor | Sung Hoon Noh | - |
dc.contributor.googleauthor | Hyun Cheol Chung | - |
dc.contributor.googleauthor | Sun Young Rha | - |
dc.identifier.doi | OAK-2005-04832 | - |
dc.contributor.localId | A01281 | - |
dc.contributor.localId | A01316 | - |
dc.contributor.localId | A02288 | - |
dc.contributor.localId | A03773 | - |
dc.contributor.localId | A03794 | - |
dc.contributor.localId | A04382 | - |
dc.relation.journalcode | J01132 | - |
dc.identifier.eissn | 1791-244X | - |
dc.identifier.pmid | 16211255 | - |
dc.identifier.url | http://www.spandidos-publications.com/ijmm/16/5/857 | - |
dc.subject.keyword | 16211255 | - |
dc.contributor.alternativeName | Noh, Sung Hoon | - |
dc.contributor.alternativeName | Rha, Sun Young | - |
dc.contributor.alternativeName | Yang, Sang Hwa | - |
dc.contributor.alternativeName | Chung, Hyun Cheol | - |
dc.contributor.alternativeName | Jeung, Hei Cheul | - |
dc.contributor.alternativeName | Hyung, Woo Jin | - |
dc.citation.volume | 16 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 857 | - |
dc.citation.endPage | 863 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, Vol.16(5) : 857-863, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 44250 | - |
dc.type.rims | ART | - |
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