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Prevention of reactive oxygen species-induced oxidative stress in human microvascular endothelial cells by green tea polyphenol

Authors
 Dong Kyun Rah  ;  Dong-Wook Han  ;  Hyun Sook Baek  ;  Suong-Hyu Hyon  ;  Jong-Chul Park 
Citation
 TOXICOLOGY LETTERS, Vol.155(2) : 269-275, 2005 
Journal Title
 TOXICOLOGY LETTERS 
ISSN
 0378-4274 
Issue Date
2005
MeSH
Cell Line ; Cell Survival/drug effects ; Endothelial Cells/drug effects* ; Endothelial Cells/metabolism ; Flavonoids/isolation & purification ; Flavonoids/pharmacology* ; Humans ; Hydrogen Peroxide/toxicity ; Oxidative Stress/drug effects* ; Phenols/isolation & purification ; Phenols/pharmacology* ; Plant Extracts/isolation & purification ; Plant Extracts/pharmacology ; Polyphenols ; Reactive Oxygen Species/toxicity* ; Tea/chemistry ; Umbilical Veins/cytology ; Xanthine Oxidase/toxicity ; Xanthines/toxicity
Keywords
Green tea polyphenols ; Reactive oxygen species ; Human microvascular endothelial cell ; Oxidative stress ; Viability
Abstract
The potential protective roles played by green tea polyphenol (GTP) against the injurious effects of reactive oxygen species in human microvascular endothelial cells (HUMVECs) were investigated. Oxidative stress was induced in cultured HUMVECs, either by adding 10 mM H2O2 or by the action of 10 U/l xanthine oxidase (XO) in the presence of xanthine (250 μM). Both treatments produced a significant reduction (to 68% and 71%, respectively) in HUMVEC viability, as assessed by fluorescence double staining method followed by flow cytometric analysis. On the microscopic observations, the morphological changes and necrotic detachment were appreciably induced by both treatments. The H2O2-induced alterations were completely prevented by pre-incubating the ECs with 10 μg/ml GTP for 1 h. When the oxidative stress was induced by XO, the cell viability and morphology were also significantly maintained at the same GTP concentration. These results demonstrate that GTP can act as a biological antioxidant in a cell culture experimental model and prevent oxidative stress-induced cytotoxicity in ECs.
Full Text
http://www.sciencedirect.com/science/article/pii/S0378427404004825
DOI
10.1016/j.toxlet.2004.10.002
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Rah, Dong Kyun(나동균)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Baek, Hyun Sook(백현숙)
Han, Dong Wook(한동욱)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/147542
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