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Prevention of reactive oxygen species-induced oxidative stress in human microvascular endothelial cells by green tea polyphenol

DC Field Value Language
dc.contributor.author나동균-
dc.contributor.author박종철-
dc.contributor.author백현숙-
dc.contributor.author한동욱-
dc.date.accessioned2017-05-04T07:36:51Z-
dc.date.available2017-05-04T07:36:51Z-
dc.date.issued2005-
dc.identifier.issn0378-4274-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/147542-
dc.description.abstractThe potential protective roles played by green tea polyphenol (GTP) against the injurious effects of reactive oxygen species in human microvascular endothelial cells (HUMVECs) were investigated. Oxidative stress was induced in cultured HUMVECs, either by adding 10 mM H2O2 or by the action of 10 U/l xanthine oxidase (XO) in the presence of xanthine (250 μM). Both treatments produced a significant reduction (to 68% and 71%, respectively) in HUMVEC viability, as assessed by fluorescence double staining method followed by flow cytometric analysis. On the microscopic observations, the morphological changes and necrotic detachment were appreciably induced by both treatments. The H2O2-induced alterations were completely prevented by pre-incubating the ECs with 10 μg/ml GTP for 1 h. When the oxidative stress was induced by XO, the cell viability and morphology were also significantly maintained at the same GTP concentration. These results demonstrate that GTP can act as a biological antioxidant in a cell culture experimental model and prevent oxidative stress-induced cytotoxicity in ECs.-
dc.description.statementOfResponsibilityrestriction-
dc.format.extent269~275-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfTOXICOLOGY LETTERS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHCell Line-
dc.subject.MESHCell Survival/drug effects-
dc.subject.MESHEndothelial Cells/drug effects*-
dc.subject.MESHEndothelial Cells/metabolism-
dc.subject.MESHFlavonoids/isolation & purification-
dc.subject.MESHFlavonoids/pharmacology*-
dc.subject.MESHHumans-
dc.subject.MESHHydrogen Peroxide/toxicity-
dc.subject.MESHOxidative Stress/drug effects*-
dc.subject.MESHPhenols/isolation & purification-
dc.subject.MESHPhenols/pharmacology*-
dc.subject.MESHPlant Extracts/isolation & purification-
dc.subject.MESHPlant Extracts/pharmacology-
dc.subject.MESHPolyphenols-
dc.subject.MESHReactive Oxygen Species/toxicity*-
dc.subject.MESHTea/chemistry-
dc.subject.MESHUmbilical Veins/cytology-
dc.subject.MESHXanthine Oxidase/toxicity-
dc.subject.MESHXanthines/toxicity-
dc.titlePrevention of reactive oxygen species-induced oxidative stress in human microvascular endothelial cells by green tea polyphenol-
dc.typeArticle-
dc.publisher.locationNetherlands-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeOthers-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Plastic and Reconstructive Surgery (성형외과학교실)-
dc.contributor.departmentDept. of Medical Engineering (의학공학교실)-
dc.contributor.departmentSeverance Hospital (세브란스병원)-
dc.contributor.departmentDept. of Medical Engineering (의학공학교실)-
dc.contributor.googleauthorDong Kyun Rah-
dc.contributor.googleauthorDong-Wook Han-
dc.contributor.googleauthorHyun Sook Baek-
dc.contributor.googleauthorSuong-Hyu Hyon-
dc.contributor.googleauthorJong-Chul Park-
dc.identifier.doi10.1016/j.toxlet.2004.10.002-
dc.contributor.localIdA01229-
dc.contributor.localIdA01662-
dc.contributor.localIdA01843-
dc.contributor.localIdA04275-
dc.relation.journalcodeJ02744-
dc.identifier.eissn1879-3169-
dc.identifier.pmid15603922-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0378427404004825-
dc.subject.keywordGreen tea polyphenols-
dc.subject.keywordReactive oxygen species-
dc.subject.keywordHuman microvascular endothelial cell-
dc.subject.keywordOxidative stress-
dc.subject.keywordViability-
dc.contributor.alternativeNameRah, Dong Kyun-
dc.contributor.alternativeNamePark, Jong Chul-
dc.contributor.alternativeNameBaek, Hyun Sook-
dc.contributor.alternativeNameHan, Dong Wook-
dc.citation.volume155-
dc.citation.number2-
dc.citation.startPage269-
dc.citation.endPage275-
dc.identifier.bibliographicCitationTOXICOLOGY LETTERS, Vol.155(2) : 269-275, 2005-
dc.date.modified2017-05-04-
dc.identifier.rimsid40317-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers

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