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Host immune responses to antigens derived from a predominant strain of Mycobacterium tuberculosis.

Authors
 Yun-Gyoung Hur  ;  Wou Young Chung  ;  Ahreum Kim  ;  Young Sun Kim  ;  Hyon-Suk Kim  ;  Sun-Hee Jang  ;  Yeun Kim  ;  Hyeyoung Lee  ;  Kwang Joo Park  ;  Sang-Nae Cho 
Citation
 JOURNAL OF INFECTION, Vol.73(1) : 54-62, 2016 
Journal Title
 JOURNAL OF INFECTION 
ISSN
 0163-4453 
Issue Date
2016
MeSH
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antigens, Bacterial/immunology* ; Cytokines/analysis ; Disease Outbreaks ; Epitopes, T-Lymphocyte/immunology ; Female ; Humans ; Male ; Middle Aged ; Mycobacterium tuberculosis/immunology* ; Republic of Korea/epidemiology ; Tuberculosis/diagnosis ; Tuberculosis/epidemiology ; Tuberculosis/microbiology* ; Young Adult
Keywords
Beijing/K strain ; CXCL10 ; IFN-γ ; Latent TB infection ; Mycobacterium tuberculosis ; TB ; TNF-α
Abstract
OBJECTIVES: Beijing strain of Mycobacterium tuberculosis (M. tb) is characterized by a high incidence of transmission, relapse, and drug resistance. This study aimed to determine host immune responses to antigens derived from the Beijing/K strain which has been highly prevalent in tuberculosis (TB) outbreaks in South Korea. METHODS: We recruited 52 TB patients and 96 healthy subjects comprising 31 individuals with latent TB infection (LTBI) and 65 TB-naïve controls based on QuantiFERON-TB Gold In-Tube (QFT-IT) tests. Blood samples were obtained for diluted whole-blood assays, multiplex bead arrays, ELISpot assays, and HLA typing. Molecular genotyping of M. tb was performed using clinical isolates. RESULTS: Active TB and LTBI groups were differentiated by TNF-α concentrations induced by the Beijing/K strain-derived antigens, MTBK_24790 and MTBK_24800 (P < 0.001). MTBK_24800-induced IFN-γ and CXCL10 concentrations discriminated the TB-infected groups from TB-naïve controls (P < 0.001). IFN-γ-producing T cells were generated in 87.2% of TB patients in response to MTBK_24800 peptide antigens. The major immunogenic epitope was at C-terminal of the antigen, and predominantly recognized by HLA-DR4 and -DQ4. CONCLUSIONS: Measurement of IFN-γ, CXCL10, and TNF-α concentrations induced by MTBK_24790 and MTBK_24800 may contribute to improved diagnosis of TB and vaccine development in regions where the Beijing/K strain is endemic.
Full Text
http://www.sciencedirect.com/science/article/pii/S0163445316300597
DOI
10.1016/j.jinf.2016.04.032
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute for Immunology and Immunological Disease (면역질환연구소) > 1. Journal Papers
Yonsei Authors
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Cho, Sang Nae(조상래)
Hur, Yun-Gyoung(허윤경) ORCID logo https://orcid.org/0000-0003-3986-4394
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146961
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