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In vivo sensing of proteolytic activity with an NSET-based NIR fluorogenic nanosensor

Authors
 Minhee Ku  ;  Yoochan Hong  ;  Dan Heo  ;  Eugene Lee  ;  Seungyeon Hwang  ;  Jin-Suck Suh  ;  Jaemoon Yang 
Citation
 BIOSENSORS & BIOELECTRONICS, Vol.77 : 471-477, 2016 
Journal Title
 BIOSENSORS & BIOELECTRONICS 
ISSN
 0956-5663 
Issue Date
2016
MeSH
Animals ; Biomarkers, Tumor/metabolism* ; Cell Line, Tumor ; Enzyme Activation ; Fluorescence Resonance Energy Transfer/instrumentation* ; Gold/chemistry ; Humans ; Matrix Metalloproteinase 14/metabolism* ; Metal Nanoparticles/chemistry ; Mice ; Nanotubes/chemistry ; Neoplasms, Experimental/enzymology* ; Neoplasms, Experimental/secondary* ; Spectroscopy, Near-Infrared/instrumentation*
Keywords
Fluorogenic ; Gold nanorod ; Membrane type 1-matrix metalloproteinase ; Nanoparticle surface energy transfer ; Nanosensor
Abstract
Biomedical in vivo sensing methods in the near-infrared (NIR) range, which that provide relatively high photon transparency, separation from auto-fluorescence background, and extended sensitivity, are being used increasingly for non-invasive mapping and monitoring of molecular events in cancer cells. In this study, we fabricated an NIR fluorogenic nanosensor based on the nanoparticle surface energy transfer effect, by conjugation of fluorescent proteolytic enzyme-specific cleavable peptides with gold nanorods (GNRs). Membrane-anchored membrane type 1-matrix metalloproteinases (MT1-MMPs), a family of zinc-dependent proteolytic enzymes, can induce the metastatic potential of cancer cells by promoting degradation of the extracellular matrix. Therefore, sensitive detection of MT1-MMP activity can provide essential information in the clinical setting. We have applied in vivo NIR sensing to evaluate MT1-MMP activity, as an NIR imaging target, in an MT1-MMP-expressing metastatic tumor mouse model.
Full Text
http://www.sciencedirect.com/science/article/pii/S0956566315304632
DOI
10.1016/j.bios.2015.09.067
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Ku, Min Hee(구민희) ORCID logo https://orcid.org/0000-0002-1674-1474
Suh, Jin Suck(서진석) ORCID logo https://orcid.org/0000-0001-9455-9240
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/145506
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