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Comparative study on motility of the cultured fetal and neonatal dermal fibroblasts in extracellular matrix

 Jong Chul Park  ;  Bong Joo Park  ;  Hwa Suh  ;  Beyoung Yun Park  ;  Dong Kyun Rah 
 YONSEI MEDICAL JOURNAL, Vol.42(6) : 587-594, 2001 
Journal Title
Issue Date
Cell Movement ; Cells, Cultured ; Extracellular Matrix/physiology* ; Fetus/physiology ; Fibroblasts/physiology* ; Humans ; Infant, Newborn ; Skin/cytology ; Skin/embryology* ; Skin Physiological Phenomena*
Fetal fibroblast ; neonatal fibroblast ; migration ; extracellular matrix ; collagen ; fibronectin ; hyaluronic acid ; video-microscopy system
One of the differences between fetal and adult skin healing is the ability of fetal wounds heal without contraction and scar formation. Extracellular matrix (ECM) provides a substratum for cells adhesion, migration, and proliferation and can directly influence the form and function of cells. As motility is essential for many important biological events, including wound healing, inflammatory response, embryonic development, and tumor metastasis, this study was designed to compare the motilities cultured dermal fetal and neonatal fibroblasts in the extracellular matrix. The motility of cultured fetal and neonatal fibroblasts was compared using a video-microscopy system that was developed in combination with a self-designed CO2 mini-incubator. To determine migration speed, cells were viewed with a 4 × phase-contrast lens and video recorded. Images were captured using a color CCD camera and saved in 8-bit full-color mode. We found that cultured fetal fibroblasts move faster than neonatal fibroblast on type I collagen (fetal fibroblast, 15.1 µm/hr; neonatal fibroblast, 13.7 µm/hr), and in fibronectin (fetal fibroblast, 13.2 µm/hr; neonatal fibroblast, 13.0 µm/hr) and hyaluronic acid (fetal fibroblast, 11 µm/hr; neonatal fibroblast, 9.8 µm/hr).
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1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Rah, Dong Kyun(나동균)
Park, Beyoung Yun(박병윤)
Park, Bong Joo(박봉주)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Suh, Hwal(서활)
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