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The regulation of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase by autophagy in low-glycolytic hepatocellular carcinoma cells

 Jeong Yong Jeon  ;  Hyangkyu Lee  ;  Jeayeo Park  ;  Misu Lee  ;  Sae Whan Park  ;  Ji Sook Kim  ;  Milim Lee  ;  Byoungchul Cho  ;  Kyungsup Kim  ;  Augustine M.K. Choi  ;  Chun K. Kim  ;  Mijin Yun 
 Biochemical and Biophysical Research Communications, Vol.463(3) : 440-446, 2015 
Journal Title
 Biochemical and Biophysical Research Communications 
Issue Date
The glycolytic phenotype is a dominant metabolic phenomenon in cancer and is reflected in becoming aggressive. Certain hepatocellular carcinoma lack increased glycolysis and prefer to uptake acetate than glucose for metabolism. Autophagy plays a role in preserving energies and nutrients when there is limited external nutrient supply and maintains glucose level of blood though supporting gluconeogenesis in the liver. As the role of autophagy and gluconeogenesis in HCC following the glycolic activity was not clear, we cultured HCC cells with different glycolytic levels in Hank's balanced salt solution (HBSS) to induce autophagy and conducted the activity of gluconeogenesis. Both autophagy and gluconeogenesis were induced in low glycolytic HCC cells (HepG2). In glycolytic Hep3B cells, only autophagy without gluconeogenesis was induced upon starvation. When autophagy was blocked, the level of glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK) was reduced in HepG2 cells and not in Hep3B. Altogether, we investigated contribution of hepatic gluconeogenesis to the metabolic phenotype of HCC cells and the role of autophagy as a potential mechanism regulating gluconeogenesis in low glycolytic HCC.
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Nuclear Medicine (핵의학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실)
1. Journal Papers (연구논문) > 3. College of Nursing (간호대학) > Dept. of Nursing (간호학과)
Yonsei Authors
김경섭(Kim, Kyung Sup) ; 윤미진(Yun, Mi Jin) ; 이향규(Lee, Hyang Kyu) ; 전정용(Jeon, Jeong Yong) ; 조병철(Cho, Byoung Chul)
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