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Effect of FGF-2 on collagen tissue regeneration by human vertebral bone marrow stem cells

 Dong-Soo Park  ;  Jung-Chul Park  ;  Jung-Seok Lee  ;  Tae-Wan Kim  ;  Ki-Joon Kim  ;  Byung-Joo Jung  ;  Eun-Kyung Shim  ;  Eun-Young Choi  ;  So-Yon Park  ;  Kyoo-Sung Cho  ;  Chang-Sung Kim 
 STEM CELLS AND DEVELOPMENT, Vol.24(2) : 228-243, 2015 
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Adult ; Animals ; Bone Marrow Cells/cytology ; Bone Marrow Cells/metabolism* ; Cell Differentiation/drug effects* ; Cells, Cultured ; Collagen/biosynthesis* ; Female ; Fibroblast Growth Factor 2/pharmacology* ; Heterografts ; Humans ; Male ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells/cytology ; Mesenchymal Stromal Cells/metabolism* ; Mice ; Mice, SCID ; Middle Aged ; Protein-Lysine 6-Oxidase/biosynthesis ; Regeneration/drug effects* ; Spine/cytology ; Spine/metabolism*
The effects of fibroblast growth factor-2 (FGF-2) on collagen tissue regeneration by human bone marrow stem cells (hBMSCs) were investigated. hBMSCs were isolated from human vertebral body bone marrow during vertebral surgery and a population of hBMSCs with the characteristics of mesenchymal stem cells was observed. The FGF-2 treatment (5 ng/mL) affected on the colony-forming efficiency, proliferation, and in vitro differentiation of hBMSCs. Insoluble/soluble collagen and hydroxyproline synthesis was significantly enhanced in hBMSCs expanded with FGF-2 and the treatment of FGF-2 caused a reduction in the mRNA expression of collagen type I, but an increase of collagen types II and III along with lysyl oxidase family genes. Collagen formation was also examined using an in vivo assay model by transplanting hBMSCs into immunocompromised mice (n=4) and the histologic and immunohistochemical results revealed that significantly more collagen with a well-organized structure was formed by FGF-2-treated hBMSCs at 8 weeks posttransplantation (P<0.05). The DNA microarray assay demonstrated that genes related to extracellular matrix formation were significantly upregulated. To elucidate the underlying mechanism, chemical inhibitors against extracellular-signal-regulated kinase (ERK) and phosphoinositide 3-kinase (PI3K) were treated and following downstream expression was observed. Collectively, FGF-2 facilitated the collagen-producing potency of hBMSCs both in vitro and in vivo, rendering them more suitable for use in collagen regeneration in the clinical field.
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2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute for Periodontal Tissue Regeneration (치주조직재생연구소) > 1. Journal Papers
Yonsei Authors
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Park, So Yon(박소연)
Lee, Jung Seok(이중석) ORCID logo https://orcid.org/0000-0003-1276-5978
Cho, Kyoo Sung(조규성) ORCID logo https://orcid.org/0000-0002-6777-5287
Choi, Eun Young(최은영)
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