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Effect of FGF-2 on collagen tissue regeneration by human vertebral bone marrow stem cells

DC Field Value Language
dc.contributor.author김창성-
dc.contributor.author박소연-
dc.contributor.author이중석-
dc.contributor.author조규성-
dc.contributor.author최은영-
dc.date.accessioned2016-02-04T10:57:39Z-
dc.date.available2016-02-04T10:57:39Z-
dc.date.issued2015-
dc.identifier.issn1547-3287-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/139359-
dc.description.abstractThe effects of fibroblast growth factor-2 (FGF-2) on collagen tissue regeneration by human bone marrow stem cells (hBMSCs) were investigated. hBMSCs were isolated from human vertebral body bone marrow during vertebral surgery and a population of hBMSCs with the characteristics of mesenchymal stem cells was observed. The FGF-2 treatment (5 ng/mL) affected on the colony-forming efficiency, proliferation, and in vitro differentiation of hBMSCs. Insoluble/soluble collagen and hydroxyproline synthesis was significantly enhanced in hBMSCs expanded with FGF-2 and the treatment of FGF-2 caused a reduction in the mRNA expression of collagen type I, but an increase of collagen types II and III along with lysyl oxidase family genes. Collagen formation was also examined using an in vivo assay model by transplanting hBMSCs into immunocompromised mice (n=4) and the histologic and immunohistochemical results revealed that significantly more collagen with a well-organized structure was formed by FGF-2-treated hBMSCs at 8 weeks posttransplantation (P<0.05). The DNA microarray assay demonstrated that genes related to extracellular matrix formation were significantly upregulated. To elucidate the underlying mechanism, chemical inhibitors against extracellular-signal-regulated kinase (ERK) and phosphoinositide 3-kinase (PI3K) were treated and following downstream expression was observed. Collectively, FGF-2 facilitated the collagen-producing potency of hBMSCs both in vitro and in vivo, rendering them more suitable for use in collagen regeneration in the clinical field.-
dc.description.statementOfResponsibilityopen-
dc.format.extent228~243-
dc.relation.isPartOfSTEM CELLS AND DEVELOPMENT-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHAnimals-
dc.subject.MESHBone Marrow Cells/cytology-
dc.subject.MESHBone Marrow Cells/metabolism*-
dc.subject.MESHCell Differentiation/drug effects*-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCollagen/biosynthesis*-
dc.subject.MESHFemale-
dc.subject.MESHFibroblast Growth Factor 2/pharmacology*-
dc.subject.MESHHeterografts-
dc.subject.MESHHumans-
dc.subject.MESHMale-
dc.subject.MESHMesenchymal Stem Cell Transplantation-
dc.subject.MESHMesenchymal Stromal Cells/cytology-
dc.subject.MESHMesenchymal Stromal Cells/metabolism*-
dc.subject.MESHMice-
dc.subject.MESHMice, SCID-
dc.subject.MESHMiddle Aged-
dc.subject.MESHProtein-Lysine 6-Oxidase/biosynthesis-
dc.subject.MESHRegeneration/drug effects*-
dc.subject.MESHSpine/cytology-
dc.subject.MESHSpine/metabolism*-
dc.titleEffect of FGF-2 on collagen tissue regeneration by human vertebral bone marrow stem cells-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Periodontology (치주과학)-
dc.contributor.googleauthorDong-Soo Park-
dc.contributor.googleauthorJung-Chul Park-
dc.contributor.googleauthorJung-Seok Lee-
dc.contributor.googleauthorTae-Wan Kim-
dc.contributor.googleauthorKi-Joon Kim-
dc.contributor.googleauthorByung-Joo Jung-
dc.contributor.googleauthorEun-Kyung Shim-
dc.contributor.googleauthorEun-Young Choi-
dc.contributor.googleauthorSo-Yon Park-
dc.contributor.googleauthorKyoo-Sung Cho-
dc.contributor.googleauthorChang-Sung Kim-
dc.identifier.doi10.1089/scd.2014.0148-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01041-
dc.contributor.localIdA01526-
dc.contributor.localIdA03810-
dc.contributor.localIdA03185-
dc.contributor.localIdA04155-
dc.relation.journalcodeJ02684-
dc.identifier.eissn1557-8534-
dc.identifier.pmid25122057-
dc.identifier.urlhttp://online.liebertpub.com/doi/abs/10.1089/scd.2014.0148-
dc.contributor.alternativeNameKim, Chang Sung-
dc.contributor.alternativeNamePark, So Yon-
dc.contributor.alternativeNameLee, Jung Seok-
dc.contributor.alternativeNameCho, Kyoo Sung-
dc.contributor.alternativeNameChoi, Eun Young-
dc.contributor.affiliatedAuthorKim, Chang Sung-
dc.contributor.affiliatedAuthorPark, So Yon-
dc.contributor.affiliatedAuthorCho, Kyoo Sung-
dc.contributor.affiliatedAuthorLee, Jung Seok-
dc.contributor.affiliatedAuthorChoi, Eun Young-
dc.rights.accessRightsnot free-
dc.citation.volume24-
dc.citation.number2-
dc.citation.startPage228-
dc.citation.endPage243-
dc.identifier.bibliographicCitationSTEM CELLS AND DEVELOPMENT, Vol.24(2) : 228-243, 2015-
dc.identifier.rimsid39399-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Research Institute (부설연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers

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