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In vitro mesenchymal stem cell culture using calcium phosphate glass scaffold

Authors
 J. Kim  ;  J.K. Ryu  ;  Yong Keun Lee  ;  Racquel Z. LeGeros  ;  Kwang Mahn Kim  ;  Kyoung Nam Kim  ;  Chong Kwan Kim  ;  Seong Ho Choi  ;  Yeon Ung Kim  ;  Min Chul Kim 
Citation
 Key Engineering Materials, Vol.284~286 : 679-682, 2005 
Journal Title
 Key Engineering Materials 
ISSN
 1013-9826 
Issue Date
2005
MeSH
Calcium Phosphate Glass ; Mesenchymal Stem Cell (MSC) ; Scaffold
Keywords
Calcium Phosphate Glass ; Mesenchymal Stem Cell (MSC) ; Scaffold
Abstract
The purpose of this study was to evaluate the cell affinity of calcium phosphate glass scaffold in the system of CaO-CaF2-P2O5-MgO-ZnO, which is already reported that promoted the bone-like tissue formation in vitro and formed new bone in Sprague-Dawley rats. We prepared calcium phosphate glass saffolds with three-dimensionally interconnected pores of 200~500 µm. Commercial HA scaffold was employed as a control in this study. Bone marrow cells were collected from the healthy human donors and cultured within the prepared scaffolds. After 2, 4, 6, and 8 weeks, hMSCs/scaffold were fixed and stained with hematoxylin and eosin. hMSCs were continuously proliferated both in the experimental and control groups at every incubation period. The number of cells was higher in the experimental group than that of the control group, however, there was no significant difference (p>0.05). Extracellular matrices could be observed at the 2nd and 4th days in the experimental and control groups, respectively. The extracellular matrices were more abundant in the experimental group at all periods. The prepared calcium phosphate glass scaffolds are expected effective in bone tissue engineering.
Full Text
http://www.scientific.net/KEM.284-286.679
DOI
10.4028/www.scientific.net/KEM.284-286.679
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyoung Nam(김경남)
Kim, Kwang Mahn(김광만) ORCID logo https://orcid.org/0000-0002-5235-0294
Kim, Min Chul(김민철)
Kim, Chong Kwan(김종관)
Kim, Jin(김진)
Ryu, Ju Kyoung(유주경)
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114821
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