In vitro mesenchymal stem cell culture using calcium phosphate glass scaffold

Abstract

The purpose of this study was to evaluate the cell affinity of calcium phosphate glass scaffold in the system of CaO-CaF2-P2O5-MgO-ZnO, which is already reported that promoted the bone-like tissue formation in vitro and formed new bone in Sprague-Dawley rats. We prepared calcium phosphate glass saffolds with three-dimensionally interconnected pores of 200~500 µm. Commercial HA scaffold was employed as a control in this study. Bone marrow cells were collected from the healthy human donors and cultured within the prepared scaffolds. After 2, 4, 6, and 8 weeks, hMSCs/scaffold were fixed and stained with hematoxylin and eosin. hMSCs were continuously proliferated both in the experimental and control groups at every incubation period. The number of cells was higher in the experimental group than that of the control group, however, there was no significant difference (p>0.05). Extracellular matrices could be observed at the 2nd and 4th days in the experimental and control groups, respectively. The extracellular matrices were more abundant in the experimental group at all periods. The prepared calcium phosphate glass scaffolds are expected effective in bone tissue engineering.