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Peptide Mimotopes of Neisseria meningitidis Group B Capsular Polysaccharide

Authors
 Inho Park  ;  In Hong Choi  ;  Jeon Soo Shin  ;  Se Jong Kim 
Citation
 YONSEI MEDICAL JOURNAL, Vol.45(4) : 755-758, 2004 
Journal Title
 YONSEI MEDICAL JOURNAL 
ISSN
 0513-5796 
Issue Date
2004
MeSH
Amino Acid Sequence ; Animals ; Bacterial Vaccines/immunology* ; Cloning, Molecular ; Female ; Meningococcal Infections/immunology* ; Meningococcal Infections/prevention & control* ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Neisseria meningitidis, Serogroup B/genetics ; Neisseria meningitidis, Serogroup B/immunology* ; Polysaccharides, Bacterial/genetics ; Polysaccharides, Bacterial/immunology*
Keywords
Neisseria meningitidis ; peptide mimotope ; phage display ; vaccine
Abstract
The antigenic similarity between Neisseria meningitidis group B (NMGB) capsular polysaccharide (PS) and human polysialic acid (PSA) has hampered the development of a NMGB PS-based vaccine. But the possibility of a safe vaccine based on NMGB PS has been demonstrated by the existence of the NMGB PS-associated nonautoreactive epitope, which is distinct from those present on human PSA. To obtain peptide mimotopes of NMGB PS, we used HmenB3, a protective and nonautoreactive monoclonal antibody, to screen a phage library with 12 amino acids. We obtained 23 phage clones that bound to HmenB3 but not in the presence of E. coli K1 PS [which is α(2-8) -linked PSA like NMGB PS]. The clones contained 3 mimotopes and differed from previously described NMGB PS mimotopes. Immunization with a synthetic peptide of one mimotope elicited anti-NMGB antibodies in BALB/c mice. These mimotopes may be useful in the development of group B meningococcal vaccines.
Files in This Item:
T200401202.pdf Download
DOI
10.3349/ymj.2004.45.4.755
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Jong(김세종)
Park, Inho(박인호) ORCID logo https://orcid.org/0000-0003-2190-5469
Shin, Jeon Soo(신전수) ORCID logo https://orcid.org/0000-0002-8294-3234
Choi, In Hong(최인홍) ORCID logo https://orcid.org/0000-0001-9851-0137
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/112521
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