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DNA microarray-based detection of nosocomial pathogenic Pseudomonas aeruginosa and Acinetobacter baumannii

Authors
 Ki Chang Keum  ;  Seung Min Yoo  ;  Sang Yup Lee  ;  Kyung Hee Chang  ;  Nae Choon Yoo  ;  Won Min Yoo  ;  June Myung Kim  ;  Jun Yong Choi  ;  Jong Seok Kim  ;  Gene Lee 
Citation
 MOLECULAR AND CELLULAR PROBES, Vol.20(1) : 42-50, 2006 
Journal Title
 MOLECULAR AND CELLULAR PROBES 
ISSN
 0890-8508 
Issue Date
2006
MeSH
Acinetobacter baumannii/genetics* ; Cross Infection/microbiology* ; Humans ; Molecular Diagnostic Techniques* ; Oligonucleotide Array Sequence Analysis/methods* ; Pseudomonas aeruginosa/genetics* ; Reproducibility of Results
Keywords
Nosocomial pathogenP ; aeruginosaA ; baumanniiDNA microarray
Abstract
Infection by nosocomial pathogenic bacteria is increasingly becoming a major threat to the patients in the hospital. We have developed a diagnostic DNA microarray for the detection of two important nosocomial pathogens, Pseudomonas aeruginosa and Acinetobacter baumannii. The diagnostic DNA microarray contains the species-specific probes of 15mer oligonucleotides designed based on the sequences of 23S ribosomal DNA. The performance of DNA microarray in diagnosing P. aeruginosa and A. baumannii was evaluated using reference bacteria as well as clinical specimens such as blood, stool, pus, sputum, urine and cerebrospinal fluid. Using this DNA microarray, A. baumannii could be successfully detected in 11 out of 13 clinical specimens, thus giving the sensitivity of 84.6% with the specificity of 100% and the positive predictive value of 100%. P. aeruginosa could also be detected in 25 out of 26 clinical specimens, showing the sensitivity of 96.2%, the specificity of 100%, and the positive predictive value of 100%. These results suggest that two nosocomial pathogens, P. aeruginosa and A. baumannii, can be efficiently diagnosed by using the DNA microarray developed in this study.
Full Text
http://www.sciencedirect.com/science/article/pii/S0890850805000733
DOI
10.1016/j.mcp.2005.09.001
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Keum, Ki Chang(금기창) ORCID logo https://orcid.org/0000-0003-4123-7998
Kim, June Myung(김준명)
Yoo, Nae Choon(유내춘)
Yoo, Won Min(유원민)
Chang, Kyung Hee(장경희)
Choi, Jun Yong(최준용) ORCID logo https://orcid.org/0000-0002-2775-3315
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/110096
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