Cited 21 times in
DNA microarray-based detection of nosocomial pathogenic Pseudomonas aeruginosa and Acinetobacter baumannii
DC Field | Value | Language |
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dc.contributor.author | 금기창 | - |
dc.contributor.author | 김준명 | - |
dc.contributor.author | 유내춘 | - |
dc.contributor.author | 유원민 | - |
dc.contributor.author | 장경희 | - |
dc.contributor.author | 최준용 | - |
dc.date.accessioned | 2015-06-10T12:35:50Z | - |
dc.date.available | 2015-06-10T12:35:50Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0890-8508 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/110096 | - |
dc.description.abstract | Infection by nosocomial pathogenic bacteria is increasingly becoming a major threat to the patients in the hospital. We have developed a diagnostic DNA microarray for the detection of two important nosocomial pathogens, Pseudomonas aeruginosa and Acinetobacter baumannii. The diagnostic DNA microarray contains the species-specific probes of 15mer oligonucleotides designed based on the sequences of 23S ribosomal DNA. The performance of DNA microarray in diagnosing P. aeruginosa and A. baumannii was evaluated using reference bacteria as well as clinical specimens such as blood, stool, pus, sputum, urine and cerebrospinal fluid. Using this DNA microarray, A. baumannii could be successfully detected in 11 out of 13 clinical specimens, thus giving the sensitivity of 84.6% with the specificity of 100% and the positive predictive value of 100%. P. aeruginosa could also be detected in 25 out of 26 clinical specimens, showing the sensitivity of 96.2%, the specificity of 100%, and the positive predictive value of 100%. These results suggest that two nosocomial pathogens, P. aeruginosa and A. baumannii, can be efficiently diagnosed by using the DNA microarray developed in this study. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 42~50 | - |
dc.relation.isPartOf | MOLECULAR AND CELLULAR PROBES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acinetobacter baumannii/genetics* | - |
dc.subject.MESH | Cross Infection/microbiology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Molecular Diagnostic Techniques* | - |
dc.subject.MESH | Oligonucleotide Array Sequence Analysis/methods* | - |
dc.subject.MESH | Pseudomonas aeruginosa/genetics* | - |
dc.subject.MESH | Reproducibility of Results | - |
dc.title | DNA microarray-based detection of nosocomial pathogenic Pseudomonas aeruginosa and Acinetobacter baumannii | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Plastic Surgery & Reconstructive Surgery (성형외과학) | - |
dc.contributor.googleauthor | Ki Chang Keum | - |
dc.contributor.googleauthor | Seung Min Yoo | - |
dc.contributor.googleauthor | Sang Yup Lee | - |
dc.contributor.googleauthor | Kyung Hee Chang | - |
dc.contributor.googleauthor | Nae Choon Yoo | - |
dc.contributor.googleauthor | Won Min Yoo | - |
dc.contributor.googleauthor | June Myung Kim | - |
dc.contributor.googleauthor | Jun Yong Choi | - |
dc.contributor.googleauthor | Jong Seok Kim | - |
dc.contributor.googleauthor | Gene Lee | - |
dc.identifier.doi | 10.1016/j.mcp.2005.09.001 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00272 | - |
dc.contributor.localId | A00953 | - |
dc.contributor.localId | A02457 | - |
dc.contributor.localId | A02489 | - |
dc.contributor.localId | A03423 | - |
dc.contributor.localId | A04191 | - |
dc.relation.journalcode | J02246 | - |
dc.identifier.eissn | 1096-1194 | - |
dc.identifier.pmid | 16269235 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0890850805000733 | - |
dc.subject.keyword | Nosocomial pathogenP | - |
dc.subject.keyword | aeruginosaA | - |
dc.subject.keyword | baumanniiDNA microarray | - |
dc.contributor.alternativeName | Keum, Ki Chang | - |
dc.contributor.alternativeName | Kim, June Myung | - |
dc.contributor.alternativeName | Yoo, Nae Choon | - |
dc.contributor.alternativeName | Yoo, Won Min | - |
dc.contributor.alternativeName | Chang, Kyung Hee | - |
dc.contributor.alternativeName | Choi, Jun Yong | - |
dc.contributor.affiliatedAuthor | Keum, Ki Chang | - |
dc.contributor.affiliatedAuthor | Kim, June Myung | - |
dc.contributor.affiliatedAuthor | Yoo, Nae Choon | - |
dc.contributor.affiliatedAuthor | Yoo, Won Min | - |
dc.contributor.affiliatedAuthor | Chang, Kyung Hee | - |
dc.contributor.affiliatedAuthor | Choi, Jun Yong | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 20 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 42 | - |
dc.citation.endPage | 50 | - |
dc.identifier.bibliographicCitation | MOLECULAR AND CELLULAR PROBES, Vol.20(1) : 42-50, 2006 | - |
dc.identifier.rimsid | 56082 | - |
dc.type.rims | ART | - |
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