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Expression of Ca2+-dependent Synaptotagmin Isoforms in Mouse and Rat Parotid Acinar Cells

Authors
 Hae Jo  ;  Hae Mi Byun  ;  Jong-hoon Kim  ;  Min Seuk Kim  ;  Seung-hyeoi Kim  ;  Jeong Hee Hong  ;  Jeong Taeg Seo  ;  Syng-ill Lee  ;  Dong Min Shin  ;  Heung-kyu Son 
Citation
 YONSEI MEDICAL JOURNAL, Vol.47(1) : 70-77, 2006 
Journal Title
YONSEI MEDICAL JOURNAL
ISSN
 0513-5796 
Issue Date
2006
MeSH
Amylases/metabolism ; Animals ; Calcium/metabolism ; Calcium/physiology* ; Carbachol/pharmacology ; Exocytosis/drug effects ; Exocytosis/physiology ; Mice ; Muscarinic Agonists/pharmacology ; Parotid Gland/cytology ; Parotid Gland/metabolism* ; Protein Isoforms/metabolism ; Rats ; Signal Transduction ; Synaptotagmins/metabolism*
Keywords
Synaptotagmin ; calcium signaling ; exocytosis ; parotid acinar cells
Abstract
Synaptotagmin is a Ca2+ sensing protein, which triggers a fusion of synaptic vesicles in neuronal transmission. Little is known regarding the expression of Ca2+-dependent synaptotagmin isoforms and their contribution to the release of secretory vesicles in mouse and rat parotid acinar cells. We investigated a type of Ca2+-dependent synaptotagmin and Ca2+ signaling in both rat and mouse parotid acinar cells using RT-PCR, microfluorometry, and amylase assay. Mouse parotid acinar cells exhibited much more sensitive amylase release in response to muscarinic stimulation than did rat parotid acinar cells. However, transient [Ca2+]i increases and Ca2+ influx in response to muscarinic stimulation in both cells were identical, suggesting that the expression or activity of the Ca2+ sensing proteins is different. Seven Ca2+-dependent synaptotagmins, from 1 to 7, were expressed in the mouse parotid acinar cells. However, in the rat parotid acinar cells, only synaptotagmins 1, 3, 4 and 7 were expressed. These results indicate that the expression of Ca2+-dependent synaptotagmins may contribute to the release of secretory vesicles in parotid acinar cells.
Files in This Item:
T200600480.pdf Download
DOI
10.3349/ymj.2006.47.1.70
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
Yonsei Authors
Seo, Jeong Taeg(서정택) ORCID logo https://orcid.org/0000-0003-2697-0251
Son, Heung Kyu(손흥규)
Shin, Dong Min(신동민) ORCID logo https://orcid.org/0000-0001-6042-0435
Lee, Syng Ill(이승일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109339
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