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Systematic analysis of cDNA microarray-based CGH.

 Chan Hee Park  ;  Ha Jin Jeong  ;  Yeon Ho Choi  ;  Sang Cheol Kim  ;  Hei Chul Jeong  ;  Kyu Hyun Park  ;  Gui Yeon Lee  ;  Tae Soo Kim  ;  Sang Wha Yang  ;  Sung Whan Ahn  ;  Yang Seok Kim  ;  Sun Young Rha  ;  Hyun Cheol Chung 
 International Journal of Molecular Medicine, Vol.17(2) : 261-267, 2006 
Journal Title
 International Journal of Molecular Medicine 
Issue Date
Chromosomes, Human/genetics ; Female ; Genome, Human/genetics ; Humans ; In Situ Hybridization, Fluorescence ; Male ; Nucleic Acid Hybridization/methods* ; Oligonucleotide Array Sequence Analysis*
cDNA microarray-based CGH (Microarray-CGH) is a useful technique for detecting genomic aberrations with a high resolution. However, the criteria for determining a genomic alteration have not been determined. We evaluated the genome-wide measurement of copy number of each gene in normal gastric and placenta tissues with both sex-matched, direct and sex-mismatched, indirect designs using 17K cDNA microarray. The results revealed the range of genomic copy number of normal tissues to be ±0.3 of the log2 ratio (gain >0.3, loss <−0.3) in the autosomal genes with direct and indirect designs. The copy number at a gene level from the X chromosomal genes using the direct and indirect sex-mismatched designs was ±0.68 of the log2 ratio (amplification >0.68, deletion <−0.68). In summary, the suggested method can be used as a guideline for analysis of genomic aberration using a Microarray-CGH in both direct and indirect designs.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Cancer Metastasis Research Center (암전이연구센터) > 1. Journal Papers
Yonsei Authors
Rha, Sun Young(라선영) ORCID logo https://orcid.org/0000-0002-2512-4531
An, Sung Whan(안성환)
Yang, Sang Hwa(양상화)
Chung, Hyun Cheol(정현철) ORCID logo https://orcid.org/0000-0002-0920-9471
Jeung, Hei Cheul(정희철) ORCID logo https://orcid.org/0000-0003-0952-3679
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