1 389

Cited 11 times in

Comparative analysis of ABCG2-expressing and label-retaining cells in mouse submandibular gland

Authors
 Yeun-Jung Kim  ;  Hyuk-Jae Kwon  ;  Naoshi Shinozaki  ;  Sadamitsu Hashimoto  ;  Masaki Shimono  ;  Sung-Won Cho  ;  Han-Sung Jung 
Citation
 CELL AND TISSUE RESEARCH, Vol.334(1) : 47-53, 2008 
Journal Title
CELL AND TISSUE RESEARCH
ISSN
 0302-766X 
Issue Date
2008
MeSH
ATP Binding Cassette Transporter, Sub-Family G, Member 2 ; ATP-Binding Cassette Transporters/biosynthesis* ; Actins/metabolism ; Animals ; Aquaporin 5/metabolism ; Bromodeoxyuridine/chemistry ; Keratins/metabolism ; Mice ; Mice, Inbred ICR ; Stem Cells/cytology* ; Stem Cells/metabolism ; Submandibular Gland/cytology* ; Submandibular Gland/metabolism
Keywords
Label-retaining cells ; BrdU ; ABCG2 ; Progenitor cell ; Submandibular gland ; Mouse (ICR)
Abstract
The submandibular gland (SMG) is a tissue that can be regenerated in a tissue injury model and that has adult stem cells capable of self-renewal and differentiation into functional cells. We have analyzed the localization of label-retaining cells (LRCs), which are putative progenitor cells, by using the BrdU-labeling method. 5-Bromo-2'-deoxyuridine (BrdU) injection followed by a long chasing period permitted the identification of LRCs based on the slow-cycling characteristic. In order to confirm the accurate localization of LRCs, BrdU and SMG-specific markers, including aquaporin5, cytokeratin, and smooth muscle actin, were examined by double-immunofluorescence staining. We found that LRCs were distributed in the acinus, duct, myoepithelium, and connective tissue. Moreover, ABCG2 (a known stem cell marker) was used for the characterization of LRCs and the localization of cells as putative stem/progenitor cells. ABCG2-expressing cells were distributed in various regions of the SMG but did not co-localize with LRCs. We suggest that putative progenitor cells exist in various regions of the SMG and have diverse capacities to differentiate into specific cells.
Full Text
http://link.springer.com/article/10.1007%2Fs00441-008-0667-8
DOI
10.1007/s00441-008-0667-8
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Hyuk Jae(권혁제)
Kim, Yeun Jung(김연정)
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/106934
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links