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Wnt11/Fgfr1b cross-talk modulates the fate of cells in palate development

 Jong-Min Lee  ;  Jae-Young Kim  ;  Kyoung-Won Cho  ;  Min-Jung Lee  ;  Sung-Won Cho  ;  Sungwook Kwak  ;  Jinglei Cai  ;  Han-Sung Jung 
 DEVELOPMENTAL BIOLOGY, Vol.314(2) : 341-350, 2008 
Journal Title
Issue Date
Aging/physiology ; Animals ; Electroporation ; Immunohistochemistry ; In Situ Hybridization ; Maxilla/cytology ; Maxilla/growth & development ; Mice ; Mice, Inbred ICR ; Organ Culture Techniques ; Palate/cytology* ; Palate/growth & development ; Polymerase Chain Reaction ; RNA, Small Interfering/genetics ; Receptor Cross-Talk/physiology ; Receptor, Fibroblast Growth Factor, Type 1/genetics ; Receptor, Fibroblast Growth Factor, Type 1/physiology* ; Wnt Proteins/genetics ; Wnt Proteins/physiology*
Wnt11 ; Fgfr1b ; Palatogenesis ; Palatal growth ; Palatal fusion ; Cell proliferation ; Apoptosis ; z-VAD-fmk ; Wnt11 siRNA
Various cellular and molecular events underlie the elevation and fusion of the developing palate that occurs during embryonic development. This includes convergent extension, where the medial edge epithelium is intercalated into the midline epithelial seam. We examined the expression patterns of Wnt11 and Fgfr1b - which are believed to be key factors in convergent extension - in mouse palate development. Wnt-11 overexpression and beads soaked in SU5402 (an Fgfr1 inhibitor) were employed in in vitro organ cultures. The results suggested that interactions between Wnt11 and Fgfr1b are important in modulating cellular events such as cell proliferation for growth and apoptosis for fusion. Moreover, the Wnt11 siRNA results showed that Wnt11-induced apoptosis was necessary for palatal fusion. In summary, Fgfr1b induces cell proliferation in the developing palate mesenchyme so that the palate grows and contacts each palatal shelf, with negative feedback of Fgfs triggered by excessive cell proliferation then inhibiting the expression of Fgfr1b and activating the expression of Wnt11 to fuse each palate by activating apoptosis.
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2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jae Young(김재영)
Lee, Min Jung(이민정)
Lee, Jong Min(이종민) ORCID logo https://orcid.org/0000-0002-9466-7644
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Cho, Kyoung Won(조경원)
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
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