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Effects of heparin-binding epidermal growth factor-like growth factor on cell repopulation and signal transduction in periodontal ligament cells after scratch wounding in vitro

Authors
 J.S.Lee  ;  J.M.Kim  ;  E.K.Hong  ;  S.-O.Kim  ;  Y.-J.Yoo  ;  J.-H.Cha 
Citation
 JOURNAL OF PERIODONTAL RESEARCH, Vol.44(1) : 52-61, 2009 
Journal Title
 JOURNAL OF PERIODONTAL RESEARCH 
ISSN
 0022-3484 
Issue Date
2009
MeSH
Cell Proliferation/drug effects ; Cells, Cultured ; Coloring Agents ; Enzyme Activation/physiology ; Heparin/analysis ; Heparin/physiology* ; Heparin-binding EGF-like Growth Factor ; Humans ; Intercellular Signaling Peptides and Proteins/analysis ; Intercellular Signaling Peptides and Proteins/physiology* ; Mitogen-Activated Protein Kinase 1/analysis ; Mitogen-Activated Protein Kinase 3/analysis ; Periodontal Ligament/injuries* ; Periodontal Ligament/pathology ; Phosphorylation/physiology ; Proto-Oncogene Proteins c-akt/analysis ; Receptor, Epidermal Growth Factor/analysis ; Receptors, Cell Surface/analysis ; Receptors, Cell Surface/physiology* ; Signal Transduction/drug effects ; Signal Transduction/physiology* ; Tetrazolium Salts ; Thiazoles ; Wound Healing/drug effects ; Wound Healing/physiology ; p38 Mitogen-Activated Protein Kinases/analysis
Keywords
heparin‐binding epidermal growth factor‐like growth factor ; periodontal ligament cell ; cell repopulation ; wound healing ; cell signaling
Abstract
BACKGROUND AND OBJECTIVE: A growing amount of attention has been placed on periodontal regeneration and wound healing for periodontal therapy. This study was conducted in an effort to determine the effects of heparin-binding epidermal growth factor-like growth factor on cell repopulation and signal transduction in periodontal ligament cells after scratch wounding in vitro. MATERIAL AND METHODS: Human periodontal ligament cells were acquired from explant tissue of human healthy periodontal ligament. After the wounding of periodontal ligament cells, the change in expression of heparin-binding epidermal growth factor-like growth factor and epidermal growth factor receptors 1-4 mRNA was assessed. The effects of heparin-binding epidermal growth factor-like growth factor on periodontal ligament cell proliferation and repopulation were assessed in vitro via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and by photographing the injuries, respectively. Extracellular signal-regulated kinase (Erk)1/2, p38 and Akt phosphorylation was characterized via western blotting. RESULTS: Scratch wounding resulted in a significant up-regulation of heparin-binding epidermal growth factor-like growth factor mRNA expression, whereas wounding had no effect on the expression levels of epidermal growth factor receptors 1-4. Interestingly, no expression of epidermal growth factor receptors 2 and 4 was detectable prior to or after wounding. Heparin-binding epidermal growth factor-like growth factor treatment promoted the proliferation and repopulation of periodontal ligament cells. The scratch wounding also stimulated the phosphorylation of Erk1/2 and p38, but not of Akt, in periodontal ligament cells, and heparin-binding epidermal growth factor-like growth factor treatment applied after wounding amplified and extended the activations of Erk1/2 and p38, but not of Akt. Furthermore, Erk1/2 inhibition blocked the process of cell repopulation induced by heparin-binding epidermal growth factor-like growth factor, whereas the inhibition of p38 delayed the process. CONCLUSION: These results indicate that heparin-binding epidermal growth factor-like growth factor may constitute a critical factor in the wound healing of human periodontal ligament cells by a mechanism that requires the activation of Erk1/2 via specific interaction with epidermal growth factor receptor 1.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0765.2007.01062.x/abstract
DOI
10.1111/j.1600-0765.2007.01062.x
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seong Oh(김성오) ORCID logo https://orcid.org/0000-0002-8620-1377
Kim, Jin Moon(김진문)
Yoo, Yun Jung(유윤정) ORCID logo https://orcid.org/0000-0002-0045-9597
Lee, Jeong Soon(이정순)
Cha, Jung Heon(차정헌) ORCID logo https://orcid.org/0000-0002-9385-2653
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104570
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