Cited 2 times in
Effects of heparin-binding epidermal growth factor-like growth factor on cell repopulation and signal transduction in periodontal ligament cells after scratch wounding in vitro
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김성오 | - |
dc.contributor.author | 김진문 | - |
dc.contributor.author | 유윤정 | - |
dc.contributor.author | 이정순 | - |
dc.contributor.author | 차정헌 | - |
dc.date.accessioned | 2015-04-24T16:59:59Z | - |
dc.date.available | 2015-04-24T16:59:59Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0022-3484 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/104570 | - |
dc.description.abstract | BACKGROUND AND OBJECTIVE: A growing amount of attention has been placed on periodontal regeneration and wound healing for periodontal therapy. This study was conducted in an effort to determine the effects of heparin-binding epidermal growth factor-like growth factor on cell repopulation and signal transduction in periodontal ligament cells after scratch wounding in vitro. MATERIAL AND METHODS: Human periodontal ligament cells were acquired from explant tissue of human healthy periodontal ligament. After the wounding of periodontal ligament cells, the change in expression of heparin-binding epidermal growth factor-like growth factor and epidermal growth factor receptors 1-4 mRNA was assessed. The effects of heparin-binding epidermal growth factor-like growth factor on periodontal ligament cell proliferation and repopulation were assessed in vitro via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and by photographing the injuries, respectively. Extracellular signal-regulated kinase (Erk)1/2, p38 and Akt phosphorylation was characterized via western blotting. RESULTS: Scratch wounding resulted in a significant up-regulation of heparin-binding epidermal growth factor-like growth factor mRNA expression, whereas wounding had no effect on the expression levels of epidermal growth factor receptors 1-4. Interestingly, no expression of epidermal growth factor receptors 2 and 4 was detectable prior to or after wounding. Heparin-binding epidermal growth factor-like growth factor treatment promoted the proliferation and repopulation of periodontal ligament cells. The scratch wounding also stimulated the phosphorylation of Erk1/2 and p38, but not of Akt, in periodontal ligament cells, and heparin-binding epidermal growth factor-like growth factor treatment applied after wounding amplified and extended the activations of Erk1/2 and p38, but not of Akt. Furthermore, Erk1/2 inhibition blocked the process of cell repopulation induced by heparin-binding epidermal growth factor-like growth factor, whereas the inhibition of p38 delayed the process. CONCLUSION: These results indicate that heparin-binding epidermal growth factor-like growth factor may constitute a critical factor in the wound healing of human periodontal ligament cells by a mechanism that requires the activation of Erk1/2 via specific interaction with epidermal growth factor receptor 1. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 52~61 | - |
dc.relation.isPartOf | JOURNAL OF PERIODONTAL RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Proliferation/drug effects | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Coloring Agents | - |
dc.subject.MESH | Enzyme Activation/physiology | - |
dc.subject.MESH | Heparin/analysis | - |
dc.subject.MESH | Heparin/physiology* | - |
dc.subject.MESH | Heparin-binding EGF-like Growth Factor | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Intercellular Signaling Peptides and Proteins/analysis | - |
dc.subject.MESH | Intercellular Signaling Peptides and Proteins/physiology* | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 1/analysis | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 3/analysis | - |
dc.subject.MESH | Periodontal Ligament/injuries* | - |
dc.subject.MESH | Periodontal Ligament/pathology | - |
dc.subject.MESH | Phosphorylation/physiology | - |
dc.subject.MESH | Proto-Oncogene Proteins c-akt/analysis | - |
dc.subject.MESH | Receptor, Epidermal Growth Factor/analysis | - |
dc.subject.MESH | Receptors, Cell Surface/analysis | - |
dc.subject.MESH | Receptors, Cell Surface/physiology* | - |
dc.subject.MESH | Signal Transduction/drug effects | - |
dc.subject.MESH | Signal Transduction/physiology* | - |
dc.subject.MESH | Tetrazolium Salts | - |
dc.subject.MESH | Thiazoles | - |
dc.subject.MESH | Wound Healing/drug effects | - |
dc.subject.MESH | Wound Healing/physiology | - |
dc.subject.MESH | p38 Mitogen-Activated Protein Kinases/analysis | - |
dc.title | Effects of heparin-binding epidermal growth factor-like growth factor on cell repopulation and signal transduction in periodontal ligament cells after scratch wounding in vitro | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | J.S.Lee | - |
dc.contributor.googleauthor | J.M.Kim | - |
dc.contributor.googleauthor | E.K.Hong | - |
dc.contributor.googleauthor | S.-O.Kim | - |
dc.contributor.googleauthor | Y.-J.Yoo | - |
dc.contributor.googleauthor | J.-H.Cha | - |
dc.identifier.doi | 10.1111/j.1600-0765.2007.01062.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00577 | - |
dc.contributor.localId | A01014 | - |
dc.contributor.localId | A02490 | - |
dc.contributor.localId | A03111 | - |
dc.contributor.localId | A04007 | - |
dc.relation.journalcode | J01696 | - |
dc.identifier.eissn | 1600-0765 | - |
dc.identifier.pmid | 18973536 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0765.2007.01062.x/abstract | - |
dc.subject.keyword | heparin‐binding epidermal growth factor‐like growth factor | - |
dc.subject.keyword | periodontal ligament cell | - |
dc.subject.keyword | cell repopulation | - |
dc.subject.keyword | wound healing | - |
dc.subject.keyword | cell signaling | - |
dc.contributor.alternativeName | Kim, Seong Oh | - |
dc.contributor.alternativeName | Kim, Jin Moon | - |
dc.contributor.alternativeName | Yoo, Yun Jung | - |
dc.contributor.alternativeName | Lee, Jeong Soon | - |
dc.contributor.alternativeName | Cha, Jung Heon | - |
dc.contributor.affiliatedAuthor | Kim, Seong Oh | - |
dc.contributor.affiliatedAuthor | Kim, Jin Moon | - |
dc.contributor.affiliatedAuthor | Yoo, Yun Jung | - |
dc.contributor.affiliatedAuthor | Lee, Jeong Soon | - |
dc.contributor.affiliatedAuthor | Cha, Jung Heon | - |
dc.citation.volume | 44 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 52 | - |
dc.citation.endPage | 61 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PERIODONTAL RESEARCH, Vol.44(1) : 52-61, 2009 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.