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Nanomechanical in situ monitoring of proteolysis of peptide by Cathepsin B

Authors
 Taeyun Kwon  ;  Jinsung Park  ;  Jaemoon Yang  ;  Dae Sung Yoon  ;  Sungsoo Na  ;  Chang-Wan Kim  ;  Jin-Suck Suh  ;  Yong-Min Huh  ;  Seungjoo Haam  ;  Kilho Eom 
Citation
 PLOS ONE, Vol.4(7) : e6248, 2009 
Journal Title
PLOS ONE
Issue Date
2009
MeSH
Cathepsin B/metabolism* ; Hydrolysis ; Kinetics ; Nanotechnology* ; Peptides/metabolism* ; Polyethylene Glycols/metabolism
Abstract
Characterization and control of proteolysis of peptides by specific cellular protease is a priori requisite for effective drug discovery. Here, we report the nanomechanical, in situ monitoring of proteolysis of peptide chain attributed to protease (Cathepsin B) by using a resonant nanomechanical microcantilever immersed in a liquid. Specifically, the detection is based on measurement of resonant frequency shift arising from proteolysis of peptides (leading to decrease of cantilever's overall mass, and consequently, increases in the resonance). It is shown that resonant microcantilever enables the quantification of proteolysis efficacy with respect to protease concentration. Remarkably, the nanomechanical, in situ monitoring of proteolysis allows us to gain insight into the kinetics of proteolysis of peptides, which is well depicted by Langmuir kinetic model. This implies that nanomechanical biosensor enables the characterization of specific cellular protease such as its kinetics.
Files in This Item:
T200902599.pdf Download
DOI
10.1371/journal.pone.0006248
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Suh, Jin Suck(서진석) ORCID logo https://orcid.org/0000-0001-9455-9240
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104346
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