Cited 25 times in
Nanomechanical in situ monitoring of proteolysis of peptide by Cathepsin B
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 서진석 | - |
dc.contributor.author | 양재문 | - |
dc.contributor.author | 허용민 | - |
dc.date.accessioned | 2015-04-24T16:52:47Z | - |
dc.date.available | 2015-04-24T16:52:47Z | - |
dc.date.issued | 2009 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/104346 | - |
dc.description.abstract | Characterization and control of proteolysis of peptides by specific cellular protease is a priori requisite for effective drug discovery. Here, we report the nanomechanical, in situ monitoring of proteolysis of peptide chain attributed to protease (Cathepsin B) by using a resonant nanomechanical microcantilever immersed in a liquid. Specifically, the detection is based on measurement of resonant frequency shift arising from proteolysis of peptides (leading to decrease of cantilever's overall mass, and consequently, increases in the resonance). It is shown that resonant microcantilever enables the quantification of proteolysis efficacy with respect to protease concentration. Remarkably, the nanomechanical, in situ monitoring of proteolysis allows us to gain insight into the kinetics of proteolysis of peptides, which is well depicted by Langmuir kinetic model. This implies that nanomechanical biosensor enables the characterization of specific cellular protease such as its kinetics. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | e6248 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cathepsin B/metabolism* | - |
dc.subject.MESH | Hydrolysis | - |
dc.subject.MESH | Kinetics | - |
dc.subject.MESH | Nanotechnology* | - |
dc.subject.MESH | Peptides/metabolism* | - |
dc.subject.MESH | Polyethylene Glycols/metabolism | - |
dc.title | Nanomechanical in situ monitoring of proteolysis of peptide by Cathepsin B | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiology (영상의학) | - |
dc.contributor.googleauthor | Taeyun Kwon | - |
dc.contributor.googleauthor | Jinsung Park | - |
dc.contributor.googleauthor | Jaemoon Yang | - |
dc.contributor.googleauthor | Dae Sung Yoon | - |
dc.contributor.googleauthor | Sungsoo Na | - |
dc.contributor.googleauthor | Chang-Wan Kim | - |
dc.contributor.googleauthor | Jin-Suck Suh | - |
dc.contributor.googleauthor | Yong-Min Huh | - |
dc.contributor.googleauthor | Seungjoo Haam | - |
dc.contributor.googleauthor | Kilho Eom | - |
dc.identifier.doi | 10.1371/journal.pone.0006248 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01916 | - |
dc.contributor.localId | A04359 | - |
dc.contributor.localId | A02315 | - |
dc.relation.journalcode | J02540 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.pmid | 19606222 | - |
dc.contributor.alternativeName | Suh, Jin Suck | - |
dc.contributor.alternativeName | Yang, Jae Moon | - |
dc.contributor.alternativeName | Huh, Yong Min | - |
dc.contributor.affiliatedAuthor | Suh, Jin Suck | - |
dc.contributor.affiliatedAuthor | Huh, Yong Min | - |
dc.contributor.affiliatedAuthor | Yang, Jae Moon | - |
dc.citation.volume | 4 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | e6248 | - |
dc.identifier.bibliographicCitation | PLOS ONE, Vol.4(7) : e6248, 2009 | - |
dc.identifier.rimsid | 52609 | - |
dc.type.rims | ART | - |
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