4 262

Cited 14 times in

Determination of lamotrigine in human serum by high-performance liquid chromatography–tandem mass spectrometry.

Authors
 Woonhyoung Lee  ;  Jeong-Ho Kim  ;  Hyon-Suk Kim  ;  Oh Hun Kwon  ;  Byung In Lee  ;  Kyoung Heo 
Citation
 NEUROLOGICAL SCIENCES, Vol.31(6) : 717-720, 2010 
Journal Title
 NEUROLOGICAL SCIENCES 
ISSN
 1590-1874 
Issue Date
2010
MeSH
Anticonvulsants/blood ; Anticonvulsants/pharmacokinetics ; Antimanic Agents/blood ; Antimanic Agents/pharmacokinetics ; Chromatography, High Pressure Liquid/methods ; Drug Monitoring/methods ; Epilepsy/drug therapy ; Humans ; Reproducibility of Results ; Sensitivity and Specificity ; Tandem Mass Spectrometry/methods* ; Triazines/blood* ; Triazines/pharmacokinetics
Keywords
Lamotrigine ; Tandem mass spectrometry ; High pressure liquid chromatography ; Epilepsy ; Drug monitoring
Abstract
A rapid and sensitive liquid chromatography–tandem mass spectrometry method was developed and validated for quantification of lamotrigine in human serum. After a simple protein precipitation using acetonitrile, the analytes were separated on a Shideido 150 mm × 2.0 mm, 5 μm Capcell Pak C18 MG column using 70% acetonitrileas mobile phase at a flow rate of 200 μl/min. Lamotrigine was eluted at 1.98 min, ionized using electrospray ionization source, and then detected by multiple reaction monitoring mode. The precursor to product ion transitions of m/z 256.1–109.0 was used to quantify. The analytical measurement range is from 0.1 to 20 μg/ml and the upper clinical reportable range is chosen to be 100 μg/ml. The method was successfully employed in a clinical application
Full Text
http://link.springer.com/article/10.1007%2Fs10072-010-0257-x
DOI
10.1007/s10072-010-0257-x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurology (신경과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Oh Hun(권오헌)
Kim, Jeong Ho(김정호) ORCID logo https://orcid.org/0000-0003-2479-0548
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Lee, Byung In(이병인)
Lee, Woon Hyoung(이운형)
Heo, Kyoung(허경)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102352
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse