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Down-regulated genes in mouse dental papillae and pulp

Authors
 H. Sasaki  ;  T. Muramatsu  ;  H.-J. Kwon  ;  H. Yamamoto  ;  S. Hashimoto  ;  H.-S. Jung  ;  M. Shimono 
Citation
 JOURNAL OF DENTAL RESEARCH, Vol.89(7) : 679-683, 2010 
Journal Title
JOURNAL OF DENTAL RESEARCH
ISSN
 0022-0345 
Issue Date
2010
MeSH
ADAM Proteins/analysis ; ADAM Proteins/genetics ; ADAMTS4 Protein ; Aldehyde Dehydrogenase/analysis ; Aldehyde Dehydrogenase/genetics ; Animals ; Cell Death/genetics ; Dental Papilla/cytology* ; Dental Papilla/embryology ; Dental Pulp/cytology* ; Dental Pulp/embryology ; Down-Regulation/genetics* ; Epithelial Cells/cytology ; Gene Expression Regulation, Developmental/genetics ; In Situ Hybridization ; Lymphoid Enhancer-Binding Factor 1/analysis ; Lymphoid Enhancer-Binding Factor 1/genetics ; Mice ; Mice, Inbred ICR ; Odontoblasts/cytology ; Odontogenesis/genetics* ; Oligonucleotide Array Sequence Analysis ; Polymerase Chain Reaction ; Procollagen N-Endopeptidase/analysis ; Procollagen N-Endopeptidase/genetics ; Tooth Calcification/genetics ; Tooth Germ/cytology ; Tooth Germ/embryology
Keywords
dental papilla ; dental pulp ; odontogenesis ; gene expression ; tooth morphogenesis
Abstract
Important factors involved in odontogenesis in mouse dental papillae disappear between the pre- and post-natal stages of development. Therefore, we hypothesized that certain genes involved in odontogenesis in dental papillae were subject to pre-/post-natal down-regulation. Our goal was to identify, by microarray analysis, which genes were down-regulated. Dental papillae were isolated from embryonic 16-day-, 18-day- (E16, E18), and post-natal 3-day-old (P3) murine first mandibular molar germs and analyzed by microarray. The number of down-regulated genes was 2269 between E16 and E18, and 3130 between E18 and P3. Drastic down-regulation (fold change > 10.0) of Adamts4, Aldha1a2, and Lef1 was observed at both E16 and E18, and quantitative RT-PCR revealed a post-natal reduction in their expression (Adamts4, 1/3; Aldh1a2, 1/13; and Lef1, 1/37). These results suggest that down-regulation of these three genes is an important factor in normal odontogenesis in dental papillae.
Full Text
http://jdr.sagepub.com/content/89/7/679.long
DOI
10.1177/0022034510366844
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Hyuk Jae(권혁제)
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101494
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