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The effect of nicotine on the production of soluble fms-like tyrosine kinase-1 and soluble endoglin in human umbilical vein endothelial cells and trophoblasts

Authors
 JA-YOUNG KWON  ;  SANG-WOOK BAI  ;  YOUNG-GUEN KWON  ;  SE-HOON KIM  ;  CHUL HOON KIM  ;  MOUNG HWA KANG  ;  JOHN A. LINTON  ;  YONG-WON PARK 
Citation
 ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA, Vol.89(4) : 565-571, 2010 
Journal Title
 ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA 
ISSN
 0001-6349 
Issue Date
2010
MeSH
Antigens, CD/metabolism* ; Bungarotoxins/pharmacology ; Cell Line ; Cell Survival/drug effects ; Endoglin ; Endothelial Cells/drug effects ; Endothelial Cells/metabolism* ; Enzyme-Linked Immunosorbent Assay ; Female ; Ganglionic Stimulants/pharmacology* ; Humans ; Nicotine/pharmacology* ; Pregnancy ; Receptors, Cell Surface/metabolism* ; Receptors, Nicotinic/metabolism ; Time Factors ; Trophoblasts/drug effects ; Trophoblasts/metabolism* ; Umbilical Veins/cytology* ; Vascular Endothelial Growth Factor Receptor-1/metabolism* ; alpha7 Nicotinic Acetylcholine Receptor
Keywords
Nicotine ; soluble fms‐like tyrosine kinase‐1 ; soluble endoglin ; trophoblasts ; human umbilical vein endothelial cells ; alpha 7 nicotinic acetylcholine receptor ; hypertension in pregnancy
Abstract
OBJECTIVES: To evaluate the effect of nicotine on the production of soluble fms-like tyrosine kinase-1 (sFlt-1) and soluble endoglin (sEng) in human umbilical vein endothelial cells (HUVECs) and trophoblast cells, and to assess the involvement of alpha 7 nicotinic acetylcholine receptor (alpha7 nAChR) in this process. METHODS: Commercially available full-term placental trophoblasts and HUVECs derived from the umbilical cord of a normal pregnancy were used. The expression of alpha7 nAChR was assessed by immunostaining, RT-PCR, and western blotting. The expression of sFlt-1 and sEng protein in the cell media after 6 and 24 hours of treatment with nicotine was evaluated using a commercially available ELISA. To determine the involvement of alpha7 nAChR in the nicotinic effect, cells were treated with the alpha7 nAChR antagonist alpha-bungarotoxin (alpha-BGT) prior to the nicotine exposure. Levels of significance were determined using the Student's t-test and one-way ANOVA, and a p-value < 0.05 was considered significant. MAIN OUTCOME MEASURES: The levels of sFlt-1 and sEng protein were evaluated before and after the nicotine treatment with or without alpha-BGT pre-treatment. RESULTS: In trophoblast cells, a significant reduction of sFlt-1 and sEng protein was observed after 24 hours of nicotine treatment as compared to the untreated group (p = 0.002, 0.000). In HUVECs, nicotine only had a suppressive effect on the expression of sEng at 6 hours (p = 0.03); there was no effect on sFlt-1 expression. However, pre-treatment with alpha-BGT did not reverse the nicotine-induced suppressive effect on the expression of sFlt-1 and sEng in trophoblasts and HUVECs. CONCLUSIONS: Nicotine reduced the production of sFlt-1 and sEng in trophoblasts and sEng in HUVECs. This effect was not mediated by alpha7 nAChR.
Full Text
http://onlinelibrary.wiley.com/doi/10.3109/00016341003692253/abstract
DOI
10.3109/00016341003692253
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Family Medicine (가정의학교실) > 1. Journal Papers
Yonsei Authors
Kang, Myung Hwa(강명화)
Kwon, Ja Young(권자영) ORCID logo https://orcid.org/0000-0003-3009-6325
Kim, Se Hoon(김세훈) ORCID logo https://orcid.org/0000-0001-7516-7372
Kim, Chul Hoon(김철훈) ORCID logo https://orcid.org/0000-0002-7360-429X
Park, Yong Won(박용원)
Bai, Sang Wook(배상욱) ORCID logo https://orcid.org/0000-0001-7724-7552
Linton, John A.(인요한) ORCID logo https://orcid.org/0000-0001-8000-3049
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101067
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