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The effect of nicotine on the production of soluble fms-like tyrosine kinase-1 and soluble endoglin in human umbilical vein endothelial cells and trophoblasts

DC FieldValueLanguage
dc.contributor.author김세훈-
dc.contributor.author김철훈-
dc.contributor.author박용원-
dc.contributor.author배상욱-
dc.contributor.author인요한-
dc.contributor.author강명화-
dc.contributor.author권자영-
dc.date.accessioned2015-04-23T16:42:14Z-
dc.date.available2015-04-23T16:42:14Z-
dc.date.issued2010-
dc.identifier.issn0001-6349-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/101067-
dc.description.abstractOBJECTIVES: To evaluate the effect of nicotine on the production of soluble fms-like tyrosine kinase-1 (sFlt-1) and soluble endoglin (sEng) in human umbilical vein endothelial cells (HUVECs) and trophoblast cells, and to assess the involvement of alpha 7 nicotinic acetylcholine receptor (alpha7 nAChR) in this process. METHODS: Commercially available full-term placental trophoblasts and HUVECs derived from the umbilical cord of a normal pregnancy were used. The expression of alpha7 nAChR was assessed by immunostaining, RT-PCR, and western blotting. The expression of sFlt-1 and sEng protein in the cell media after 6 and 24 hours of treatment with nicotine was evaluated using a commercially available ELISA. To determine the involvement of alpha7 nAChR in the nicotinic effect, cells were treated with the alpha7 nAChR antagonist alpha-bungarotoxin (alpha-BGT) prior to the nicotine exposure. Levels of significance were determined using the Student's t-test and one-way ANOVA, and a p-value < 0.05 was considered significant. MAIN OUTCOME MEASURES: The levels of sFlt-1 and sEng protein were evaluated before and after the nicotine treatment with or without alpha-BGT pre-treatment. RESULTS: In trophoblast cells, a significant reduction of sFlt-1 and sEng protein was observed after 24 hours of nicotine treatment as compared to the untreated group (p = 0.002, 0.000). In HUVECs, nicotine only had a suppressive effect on the expression of sEng at 6 hours (p = 0.03); there was no effect on sFlt-1 expression. However, pre-treatment with alpha-BGT did not reverse the nicotine-induced suppressive effect on the expression of sFlt-1 and sEng in trophoblasts and HUVECs. CONCLUSIONS: Nicotine reduced the production of sFlt-1 and sEng in trophoblasts and sEng in HUVECs. This effect was not mediated by alpha7 nAChR.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAntigens, CD/metabolism*-
dc.subject.MESHBungarotoxins/pharmacology-
dc.subject.MESHCell Line-
dc.subject.MESHCell Survival/drug effects-
dc.subject.MESHEndoglin-
dc.subject.MESHEndothelial Cells/drug effects-
dc.subject.MESHEndothelial Cells/metabolism*-
dc.subject.MESHEnzyme-Linked Immunosorbent Assay-
dc.subject.MESHFemale-
dc.subject.MESHGanglionic Stimulants/pharmacology*-
dc.subject.MESHHumans-
dc.subject.MESHNicotine/pharmacology*-
dc.subject.MESHPregnancy-
dc.subject.MESHReceptors, Cell Surface/metabolism*-
dc.subject.MESHReceptors, Nicotinic/metabolism-
dc.subject.MESHTime Factors-
dc.subject.MESHTrophoblasts/drug effects-
dc.subject.MESHTrophoblasts/metabolism*-
dc.subject.MESHUmbilical Veins/cytology*-
dc.subject.MESHVascular Endothelial Growth Factor Receptor-1/metabolism*-
dc.subject.MESHalpha7 Nicotinic Acetylcholine Receptor-
dc.titleThe effect of nicotine on the production of soluble fms-like tyrosine kinase-1 and soluble endoglin in human umbilical vein endothelial cells and trophoblasts-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.googleauthorJA-YOUNG KWON-
dc.contributor.googleauthorSANG-WOOK BAI-
dc.contributor.googleauthorYOUNG-GUEN KWON-
dc.contributor.googleauthorSE-HOON KIM-
dc.contributor.googleauthorCHUL HOON KIM-
dc.contributor.googleauthorMOUNG HWA KANG-
dc.contributor.googleauthorJOHN A. LINTON-
dc.contributor.googleauthorYONG-WON PARK-
dc.identifier.doi10.3109/00016341003692253-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00610-
dc.contributor.localIdA01057-
dc.contributor.localIdA01581-
dc.contributor.localIdA01793-
dc.contributor.localIdA03352-
dc.contributor.localIdA00020-
dc.contributor.localIdA00246-
dc.relation.journalcodeJ00022-
dc.identifier.eissn1600-0412-
dc.identifier.pmid20367431-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.3109/00016341003692253/abstract-
dc.subject.keywordNicotine-
dc.subject.keywordsoluble fms‐like tyrosine kinase‐1-
dc.subject.keywordsoluble endoglin-
dc.subject.keywordtrophoblasts-
dc.subject.keywordhuman umbilical vein endothelial cells-
dc.subject.keywordalpha 7 nicotinic acetylcholine receptor-
dc.subject.keywordhypertension in pregnancy-
dc.contributor.alternativeNameKim, Se Hoon-
dc.contributor.alternativeNameKim, Chul Hoon-
dc.contributor.alternativeNamePark, Yong Won-
dc.contributor.alternativeNameBai, Sang Wook-
dc.contributor.alternativeNameLinton, John A.-
dc.contributor.alternativeNameKang, Myung Hwa-
dc.contributor.alternativeNameKwon, Ja Young-
dc.contributor.affiliatedAuthorKim, Se Hoon-
dc.contributor.affiliatedAuthorKim, Chul Hoon-
dc.contributor.affiliatedAuthorPark, Yong Won-
dc.contributor.affiliatedAuthorBai, Sang Wook-
dc.contributor.affiliatedAuthorLinton, John A.-
dc.contributor.affiliatedAuthorKang, Myung Hwa-
dc.contributor.affiliatedAuthorKwon, Ja Young-
dc.citation.volume89-
dc.citation.number4-
dc.citation.startPage565-
dc.citation.endPage571-
dc.identifier.bibliographicCitationACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA, Vol.89(4) : 565-571, 2010-
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Family Medicine (가정의학교실) > 1. Journal Papers

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