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냉장보존 및 냉동보존이 성견 치주인대세포 생존능에 미치는 영향

Other Titles
 The effects of Cold Preservation and Cryopreservation on th Viability of Periodontal Ligament Cells in Dog Teeth 
Authors
 이동은  ;  정임희  ;  엄유정  ;  정의원  ;  김창성  ;  이승종  ;  최성호 
Citation
 Biomaterials Research (생체재료학회지), Vol.14(1) : 19-24, 2010 
Journal Title
Biomaterials Research(생체재료학회지)
ISSN
 1226-1226 
Issue Date
2010
Keywords
Periodontal ligament cell ; Cold preservation ; Cryopreservation ; Viability
Abstract
The purpose of this study was to evaluate the effects of cold preservation at 4oC and cryopreservation at -196oC on the viability of periodontal ligament cells in dog teeth using WST-1(4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]- 1,3-benzene disulfonate) assay. A total of 7 beagle dogs were used. Eight teeth of each group were divided into 7 experimental groups depending upon the preservation condition. The experimental groups were group 1 (immediate control), group 2-1 (cold preservation at 4oC for 3 days), group 2-2 (cold preservation at 4oC for 1 week), group 2-3 (cold preservation at 4oC for 2 week), group 3-1 (cryopreservation for 3 days), group 3-2 (cryopreservation for 1week), group 3-3 (cryopreservation for 2 weeks). F-medium and 10% dimethylsulfoxide (DMSO) were used as preservation medium and cryoprotectant. For cryopreservation groups, thawing was performed in 37oC water bath, then WST-1 assay was processed. The values of optical density obtained by WST-1 were divided by the values of eosin staining for tissue volume standardization. In WST-1 assay, all cold preservation (4oC) groups (group 2, 3, 4) showed significantly higher viability of periodontal ligament cells than cryopreservation group (group 5, 6, 7) (p < 0.05), but showed lower viability than immediate control group (p < 0.05). In cold preservation (4oC) groups, group 2 showed significantly higher viability than group 3 and 4 (p < 0.05). There was no significant difference between all cryopreservation groups (-196oC). From the results of this study, cold preservation method suggests the better efficacy for short term preservation of the teeth than cryopreservation
Files in This Item:
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Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Um, Yoo Jung(엄유정)
Lee, Seung Jong(이승종)
Jung, Ui Won(정의원) ORCID logo https://orcid.org/0000-0001-6371-4172
Jung, Im Hee(정임희)
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100996
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