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PSA-NCAM+ Neural Precursor Cells from Human Embryonic Stem Cells Promote Neural Tissue Integrity and Behavioral Performance in A Rat Stroke Model

Authors
 Han-Soo Kim  ;  Seong-Mi Choi  ;  Wonsuk Yang  ;  Dae-Sung Kim  ;  Dongjin R. Lee  ;  Sung-Rae Cho  ;  Dong-Wook Kim 
Citation
 STEM CELL REVIEWS AND REPORTS, Vol.10(6) : 761-771, 2014 
Journal Title
 STEM CELL REVIEWS AND REPORTS 
ISSN
 2629-3269 
Issue Date
2014
MeSH
Animals ; Brain/drug effects ; Brain/metabolism ; Brain/physiology ; Cell Differentiation/drug effects ; Cell Differentiation/physiology ; Disease Models, Animal ; Embryonic Stem Cells/drug effects* ; Embryonic Stem Cells/metabolism ; Embryonic Stem Cells/physiology* ; Glial Fibrillary Acidic Protein/metabolism ; Humans ; Infarction, Middle Cerebral Artery/metabolism ; Infarction, Middle Cerebral Artery/physiopathology ; Infarction, Middle Cerebral Artery/therapy ; Male ; Nerve Tissue Proteins/metabolism ; Neural Cell Adhesion Molecules/metabolism* ; Neural Stem Cells/drug effects* ; Neural Stem Cells/metabolism ; Neural Stem Cells/physiology* ; Neurons/drug effects ; Neurons/metabolism ; Neurons/physiology ; Rats ; Rats, Sprague-Dawley ; Sialic Acids/pharmacology* ; Stroke/metabolism ; Stroke/therapy*
Keywords
PSA-NCAM ; Neural precursor cells ; Human embryonic stem cells ; Pluripotent stem cells ; Mesenchymal stem cells ; Ischemic stroke
Abstract
Recently, cell-based therapy has been highlighted as an alternative to treating ischemic brain damage in stroke patients. The present study addresses the therapeutic potential of polysialic acid-neural cell adhesion molecule (PSA-NCAM)-positive neural precursor cells (NPCPSA-NCAM+) derived from human embryonic stem cells (hESCs) in a rat stroke model with permanent middle cerebral artery occlusion. Data showed that rats transplanted with NPCPSA-NCAM+ are superior to those treated with phosphate buffered saline (PBS) or mesenchymal stem cells (MSCs) in behavioral performance throughout time points. In order to investigate its underlying events, immunohistochemical analysis was performed on rat ischemic brains treated with PBS, MSCs, and NPCPSA-NCAM+. Unlike MSCs, NPCPSA-NCAM+ demonstrated a potent immunoreactivity against human specific nuclei, doublecortin, and Tuj1 at day 26 post-transplantation, implying their survival, differentiation, and integration in the host brain. Significantly, NPCPSA-NCAM+ evidently lowered the positivity of microglial ED-1 and astrocytic GFAP, suggesting a suppression of adverse glial activation in the host brain. In addition, NPCPSA-NCAM+ elevated α-SMA+ immunoreactivity and the expression of angiopoietin-1 indicating angiogenic stimulation in the host brain. Taken together, the current data demonstrate that transplanted NPCPSA-NCAM+ preserve brain tissue with reduced infarct size and improve behavioral performance through actions encompassing anti-reactive glial activation and pro-angiogenic activity in a rat stroke model. In conclusion, the present findings support the potentiality of NPCPSA-NCAM+ as the promising source in the development of cell-based therapy for neurological diseases including ischemic stroke.
Full Text
http://link.springer.com/article/10.1007%2Fs12015-014-9535-y
DOI
10.1007/s12015-014-9535-y
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Wook(김동욱) ORCID logo https://orcid.org/0000-0002-5025-1532
Yang, Won Suk(양원석)
Lee, Dongjin R.(이동진)
Cho, Sung-Rae(조성래) ORCID logo https://orcid.org/0000-0003-1429-2684
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100303
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