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PSA-NCAM+ Neural Precursor Cells from Human Embryonic Stem Cells Promote Neural Tissue Integrity and Behavioral Performance in A Rat Stroke Model

DC Field Value Language
dc.contributor.author김동욱-
dc.contributor.author양원석-
dc.contributor.author이동진-
dc.contributor.author조성래-
dc.date.accessioned2015-01-06T17:36:43Z-
dc.date.available2015-01-06T17:36:43Z-
dc.date.issued2014-
dc.identifier.issn2629-3269-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/100303-
dc.description.abstractRecently, cell-based therapy has been highlighted as an alternative to treating ischemic brain damage in stroke patients. The present study addresses the therapeutic potential of polysialic acid-neural cell adhesion molecule (PSA-NCAM)-positive neural precursor cells (NPCPSA-NCAM+) derived from human embryonic stem cells (hESCs) in a rat stroke model with permanent middle cerebral artery occlusion. Data showed that rats transplanted with NPCPSA-NCAM+ are superior to those treated with phosphate buffered saline (PBS) or mesenchymal stem cells (MSCs) in behavioral performance throughout time points. In order to investigate its underlying events, immunohistochemical analysis was performed on rat ischemic brains treated with PBS, MSCs, and NPCPSA-NCAM+. Unlike MSCs, NPCPSA-NCAM+ demonstrated a potent immunoreactivity against human specific nuclei, doublecortin, and Tuj1 at day 26 post-transplantation, implying their survival, differentiation, and integration in the host brain. Significantly, NPCPSA-NCAM+ evidently lowered the positivity of microglial ED-1 and astrocytic GFAP, suggesting a suppression of adverse glial activation in the host brain. In addition, NPCPSA-NCAM+ elevated α-SMA+ immunoreactivity and the expression of angiopoietin-1 indicating angiogenic stimulation in the host brain. Taken together, the current data demonstrate that transplanted NPCPSA-NCAM+ preserve brain tissue with reduced infarct size and improve behavioral performance through actions encompassing anti-reactive glial activation and pro-angiogenic activity in a rat stroke model. In conclusion, the present findings support the potentiality of NPCPSA-NCAM+ as the promising source in the development of cell-based therapy for neurological diseases including ischemic stroke.-
dc.description.statementOfResponsibilityopen-
dc.format.extent761~771-
dc.languageEnglish-
dc.publisherHumana Press-
dc.relation.isPartOfSTEM CELL REVIEWS AND REPORTS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBrain/drug effects-
dc.subject.MESHBrain/metabolism-
dc.subject.MESHBrain/physiology-
dc.subject.MESHCell Differentiation/drug effects-
dc.subject.MESHCell Differentiation/physiology-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHEmbryonic Stem Cells/drug effects*-
dc.subject.MESHEmbryonic Stem Cells/metabolism-
dc.subject.MESHEmbryonic Stem Cells/physiology*-
dc.subject.MESHGlial Fibrillary Acidic Protein/metabolism-
dc.subject.MESHHumans-
dc.subject.MESHInfarction, Middle Cerebral Artery/metabolism-
dc.subject.MESHInfarction, Middle Cerebral Artery/physiopathology-
dc.subject.MESHInfarction, Middle Cerebral Artery/therapy-
dc.subject.MESHMale-
dc.subject.MESHNerve Tissue Proteins/metabolism-
dc.subject.MESHNeural Cell Adhesion Molecules/metabolism*-
dc.subject.MESHNeural Stem Cells/drug effects*-
dc.subject.MESHNeural Stem Cells/metabolism-
dc.subject.MESHNeural Stem Cells/physiology*-
dc.subject.MESHNeurons/drug effects-
dc.subject.MESHNeurons/metabolism-
dc.subject.MESHNeurons/physiology-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHSialic Acids/pharmacology*-
dc.subject.MESHStroke/metabolism-
dc.subject.MESHStroke/therapy*-
dc.titlePSA-NCAM+ Neural Precursor Cells from Human Embryonic Stem Cells Promote Neural Tissue Integrity and Behavioral Performance in A Rat Stroke Model-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Rehabilitation Medicine (재활의학)-
dc.contributor.googleauthorHan-Soo Kim-
dc.contributor.googleauthorSeong-Mi Choi-
dc.contributor.googleauthorWonsuk Yang-
dc.contributor.googleauthorDae-Sung Kim-
dc.contributor.googleauthorDongjin R. Lee-
dc.contributor.googleauthorSung-Rae Cho-
dc.contributor.googleauthorDong-Wook Kim-
dc.identifier.doi10.1007/s12015-014-9535-y-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02303-
dc.contributor.localIdA02734-
dc.contributor.localIdA03831-
dc.contributor.localIdA00406-
dc.relation.journalcodeJ02682-
dc.identifier.eissn2629-3277-
dc.identifier.pmid24974101-
dc.identifier.urlhttp://link.springer.com/article/10.1007%2Fs12015-014-9535-y-
dc.subject.keywordPSA-NCAM-
dc.subject.keywordNeural precursor cells-
dc.subject.keywordHuman embryonic stem cells-
dc.subject.keywordPluripotent stem cells-
dc.subject.keywordMesenchymal stem cells-
dc.subject.keywordIschemic stroke-
dc.contributor.alternativeNameKim, Dong Wook-
dc.contributor.alternativeNameYang, Won Suk-
dc.contributor.alternativeNameLee, Dongjin R.-
dc.contributor.alternativeNameCho, Sung Rae-
dc.contributor.affiliatedAuthorYang, Won Suk-
dc.contributor.affiliatedAuthorLee, Dongjin R.-
dc.contributor.affiliatedAuthorCho, Sung Rae-
dc.contributor.affiliatedAuthorKim, Dong Wook-
dc.rights.accessRightsfree-
dc.citation.volume10-
dc.citation.number6-
dc.citation.startPage761-
dc.citation.endPage771-
dc.identifier.bibliographicCitationSTEM CELL REVIEWS AND REPORTS, Vol.10(6) : 761-771, 2014-
dc.identifier.rimsid57577-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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