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Alveolar Bone Resorption Induced by CD4+CD45RB High-Density T-Cell Transfer in Immunocompromised Mice

Authors
 Jung-Chul Park  ;  Bo-Kyoung Kim  ;  Im-Hee Jung  ;  Eunyoung Choi  ;  Chang-Sung Kim 
Citation
 JOURNAL OF PERIODONTOLOGY, Vol.85(9) : 339-347, 2014 
Journal Title
 JOURNAL OF PERIODONTOLOGY 
ISSN
 0022-3492 
Issue Date
2014
MeSH
Adipogenesis/immunology ; Adoptive Transfer/methods* ; Adult ; Alveolar Bone Loss/immunology* ; Alveolar Bone Loss/pathology ; Alveolar Process/pathology ; Animals ; Bone Density/immunology ; CD4-Positive T-Lymphocytes/immunology* ; CD4-Positive T-Lymphocytes/transplantation ; Cell Culture Techniques ; Cell Differentiation/immunology ; Cells, Cultured ; Cementogenesis/immunology ; Female ; Humans ; Immunocompromised Host* ; Inflammatory Bowel Diseases/immunology ; Leukocyte Common Antigens/immunology* ; Male ; Mandible/immunology ; Mandible/pathology ; Mesenchymal Stromal Cells/immunology ; Mice ; Mice, Inbred ICR ; Mice, SCID ; Osteogenesis/immunology ; Periodontal Ligament/cytology ; Periodontal Ligament/immunology ; Stem Cell Transplantation/methods ; Tibia/immunology ; Tibia/pathology ; X-Ray Microtomography/methods ; Young Adult
Keywords
Alveolar bone loss ; CD4-positive T-lymphocytes ; inflammatory bowel diseases
Abstract
BACKGROUND: The aims of this study are to determine whether the antigen-inexperienced (naive, CD45RB high-density) T-cell (CD4(+)CD45RB(High) T-cell) transfer model is associated with alveolar bone resorption, to elucidate the local osteogenic/adipogenic potential of alveolar bone marrow stromal cells (ABCs) from T-cell-transferred animals, and to investigate the systemic osteogenic potential by transplanting human periodontal ligament stem cells (hPDLSCs) into these animals. METHODS: CD4(+)CD45RB(High) and CD4(+)CD45RB(Low) (antigen-experienced [memory, CD45RB low-density]) T cells were sorted and transferred into severe combined immunodeficiency (SCID) mice to induce inflammatory bowel disease-like syndrome (n = 8). hPDLSCs were transplanted into T-cell-transferred SCID mice to examine ectopic cementum formation 8 weeks after T-cell transfer. The mandibles and tibias of these mice were retrieved for microcomputed tomography (micro-CT), histomorphometric analysis, and isolation of ABCs 16 weeks after T-cell transfer. The in vitro osteogenic and adipogenic potentials of the ABCs were evaluated. RESULTS: Histologic and micro-CT analysis revealed that the transfer of CD4(+)CD45RB(High) T-cell subset was sufficient for alveolar bone resorption and affected the osteogenic/adipogenic potential of ABCs. Furthermore, it was found that CD4(+)CD45RB(High) T-cell-transferred animals have decreased systemic osteogenic potential, as evidenced using the in vivo ectopic hPDLSC transplantation model. CONCLUSION: CD4(+)CD45RB(High) T-cell transfer induced both alveolar bone resorption and reduced systemic osteogenic potential, with a concomitant downregulation of the osteogenic potential of ABCs.
Full Text
http://www.joponline.org/doi/abs/10.1902/jop.2014.140077
DOI
10.1902/jop.2014.140077
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute for Periodontal Tissue Regeneration (치주조직재생연구소) > 1. Journal Papers
Yonsei Authors
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Park, Jung Chul(박정철)
Jung, Im Hee(정임희)
Choi, Eun Young(최은영)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/99984
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