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Erythropoietic Potential of CD34+ Hematopoietic Stem Cells from Human Cord Blood and G-CSF-Mobilized Peripheral Blood

Authors
 Honglian Jin  ;  Han-Soo Kim  ;  Sinyoung Kim  ;  Hyun Ok Kim 
Citation
 BIOMED RESEARCH INTERNATIONAL, Vol.2014 : 435215, 2014 
Journal Title
 BIOMED RESEARCH INTERNATIONAL 
ISSN
 2314-6133 
Issue Date
2014
MeSH
Adult ; Antigens, CD34/genetics* ; Cell Differentiation/genetics ; Fetal Blood/cytology* ; Granulocyte Colony-Stimulating Factor/chemistry ; Granulocyte Colony-Stimulating Factor/metabolism ; Hematopoietic Stem Cells/cytology* ; Humans ; Peripheral Blood Stem Cell Transplantation*
Abstract
Red blood cell (RBC) supply for transfusion has been severely constrained by the limited availability of donor blood and the emergence of infection and contamination issues. Alternatively, hematopoietic stem cells (HSCs) from human organs have been increasingly considered as safe and effective blood source. Several methods have been studied to obtain mature RBCs from CD34+ hematopoietic stem cells via in vitro culture. Among them, human cord blood (CB) and granulocyte colony-stimulating factor-mobilized adult peripheral blood (mPB) are common adult stem cells used for allogeneic transplantation. Our present study focuses on comparing CB- and mPB-derived stem cells in differentiation from CD34+ cells into mature RBCs. By using CD34+ cells from cord blood and G-CSF mobilized peripheral blood, we showed in vitro RBC generation of artificial red blood cells. Our results demonstrate that CB- and mPB-derived CD34+ hematopoietic stem cells have similar characteristics when cultured under the same conditions, but differ considerably with respect to expression levels of various genes and hemoglobin development. This study is the first to compare the characteristics of CB- and mPB-derived erythrocytes. The results support the idea that CB and mPB, despite some similarities, possess different erythropoietic potentials in in vitro culture systems.
Files in This Item:
T201403436.pdf Download
DOI
10.1155/2014/435215
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Sin Young(김신영) ORCID logo https://orcid.org/0000-0002-2609-8945
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
Jin, Hong Lian(김홍련)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/99925
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