427 515

Cited 18 times in

Erythropoietic Potential of CD34+ Hematopoietic Stem Cells from Human Cord Blood and G-CSF-Mobilized Peripheral Blood

DC Field Value Language
dc.contributor.author김신영-
dc.contributor.author김현옥-
dc.contributor.author김홍련-
dc.date.accessioned2015-01-06T17:24:36Z-
dc.date.available2015-01-06T17:24:36Z-
dc.date.issued2014-
dc.identifier.issn2314-6133-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/99925-
dc.description.abstractRed blood cell (RBC) supply for transfusion has been severely constrained by the limited availability of donor blood and the emergence of infection and contamination issues. Alternatively, hematopoietic stem cells (HSCs) from human organs have been increasingly considered as safe and effective blood source. Several methods have been studied to obtain mature RBCs from CD34+ hematopoietic stem cells via in vitro culture. Among them, human cord blood (CB) and granulocyte colony-stimulating factor-mobilized adult peripheral blood (mPB) are common adult stem cells used for allogeneic transplantation. Our present study focuses on comparing CB- and mPB-derived stem cells in differentiation from CD34+ cells into mature RBCs. By using CD34+ cells from cord blood and G-CSF mobilized peripheral blood, we showed in vitro RBC generation of artificial red blood cells. Our results demonstrate that CB- and mPB-derived CD34+ hematopoietic stem cells have similar characteristics when cultured under the same conditions, but differ considerably with respect to expression levels of various genes and hemoglobin development. This study is the first to compare the characteristics of CB- and mPB-derived erythrocytes. The results support the idea that CB and mPB, despite some similarities, possess different erythropoietic potentials in in vitro culture systems.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.relation.isPartOfBIOMED RESEARCH INTERNATIONAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHAntigens, CD34/genetics*-
dc.subject.MESHCell Differentiation/genetics-
dc.subject.MESHFetal Blood/cytology*-
dc.subject.MESHGranulocyte Colony-Stimulating Factor/chemistry-
dc.subject.MESHGranulocyte Colony-Stimulating Factor/metabolism-
dc.subject.MESHHematopoietic Stem Cells/cytology*-
dc.subject.MESHHumans-
dc.subject.MESHPeripheral Blood Stem Cell Transplantation*-
dc.titleErythropoietic Potential of CD34+ Hematopoietic Stem Cells from Human Cord Blood and G-CSF-Mobilized Peripheral Blood-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorHonglian Jin-
dc.contributor.googleauthorHan-Soo Kim-
dc.contributor.googleauthorSinyoung Kim-
dc.contributor.googleauthorHyun Ok Kim-
dc.identifier.doi10.1155/2014/435215-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00675-
dc.contributor.localIdA01122-
dc.contributor.localIdA01190-
dc.relation.journalcodeJ00315-
dc.identifier.eissn2314-6141-
dc.identifier.pmid24883313-
dc.contributor.alternativeNameKim, Sin Young-
dc.contributor.alternativeNameKim, Hyun Ok-
dc.contributor.alternativeNameJin, Hong Lian-
dc.contributor.affiliatedAuthorKim, Sin Young-
dc.contributor.affiliatedAuthorKim, Hyun Ok-
dc.contributor.affiliatedAuthorJin, Hong Lian-
dc.citation.volume2014-
dc.citation.startPage435215-
dc.identifier.bibliographicCitationBIOMED RESEARCH INTERNATIONAL, Vol.2014 : 435215, 2014-
dc.identifier.rimsid53890-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.