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Chronic Ocular Hypertensive Rat Model using Microbead Injection: Comparison of Polyurethane, Polymethylmethacrylate, Silica and Polystyene Microbeads

 Seungsoo Rho  ;  Insung Park  ;  Gong Je Seong  ;  Naeun Lee  ;  Chang-Kyu Lee  ;  Samin Hong  ;  Chan Yun Kim 
 CURRENT EYE RESEARCH, Vol.39(9) : 917-927, 2014 
Journal Title
Issue Date
Animals ; Anterior Chamber/drug effects ; Axons/pathology ; Chronic Disease ; Disease Models, Animal* ; Injections, Intraocular ; Intraocular Pressure/drug effects* ; Male ; Microspheres* ; Ocular Hypertension/etiology* ; Ocular Hypertension/physiopathology ; Polymethyl Methacrylate/chemistry ; Polystyrenes/chemistry ; Polyurethanes/chemistry ; Rats ; Rats, Sprague-Dawley ; Retinal Ganglion Cells/pathology ; Silicon Dioxide/chemistry ; Suspensions/adverse effects
Chronic ocular hypertension ; microbead ; optic nerve ; polyurethane ; rat
PURPOSE: To establish and assess an ocular hypertensive rat model using intracameral injection with various microbeads of different sizes and materials. METHODS: Chronic elevation of intraocular pressure (IOP) was induced by the injection of various microbeads into the anterior chamber of Sprague-Dawley rat eyes. We compared the IOPs induced by the injection of different microbeads [7- and 17-µm polyurethane (PU), 7- and 15-µm polymethylmethacrylate (PMMA), 13-µm silica, and 15-µm polystyrene (PS)] and selected the appropriate microbeads for a chronic ocular hypertensive model in terms of IOP elevation and adverse events. IOP changes were observed for 4 weeks after microbead injections. Axonal degeneration was assessed with transmission electron microscopic photographs and RGC loss was assessed with retrograde labeling. RESULTS: Seventy-eight rats were included. Three days after a single injection of microbeads, IOPs were increased by 24.0% by 7-µm PU microbeads, 101.8% by 17-µm PU microbeads, 56.6% by 7-µm PMMA microbeads, 22.0% by 15-µm PMMA microbeads, 153.0% by 13-µm silica microbeads, and 34.7% by 15-µm PS microbeads. 17-µm PU microbeads produced constant IOP elevation with good reproducibility (standard deviation of <6.5 mmHg). Silica injected eyes showed severe inflammation. Sustained IOP elevation by two injections of 17-µm PU microbeads resulted in a 42% axon loss and 36.5% RGC loss (p < 0.05, Mann-Whitney U test). CONCLUSIONS: PU microbead injections offer an applicable and versatile model for a chronic ocular hypertensive model in rats. Among several biomaterials, PU microbeads produced a more stable IOP elevation without adverse events.
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1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Chan Yun(김찬윤) ORCID logo https://orcid.org/0000-0002-8373-9999
Park, In Sung(박인성)
Seong, Gong Je(성공제) ORCID logo https://orcid.org/0000-0002-5456-4296
Lee, Na Eun(이나은)
Hong, Sa Min(홍사민)
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