Cited 10 times in
Chronic Ocular Hypertensive Rat Model using Microbead Injection: Comparison of Polyurethane, Polymethylmethacrylate, Silica and Polystyene Microbeads
DC Field | Value | Language |
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dc.contributor.author | 홍사민 | - |
dc.contributor.author | 김찬윤 | - |
dc.contributor.author | 박인성 | - |
dc.contributor.author | 성공제 | - |
dc.contributor.author | 이나은 | - |
dc.date.accessioned | 2015-01-06T17:18:50Z | - |
dc.date.available | 2015-01-06T17:18:50Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0271-3683 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/99745 | - |
dc.description.abstract | PURPOSE: To establish and assess an ocular hypertensive rat model using intracameral injection with various microbeads of different sizes and materials. METHODS: Chronic elevation of intraocular pressure (IOP) was induced by the injection of various microbeads into the anterior chamber of Sprague-Dawley rat eyes. We compared the IOPs induced by the injection of different microbeads [7- and 17-µm polyurethane (PU), 7- and 15-µm polymethylmethacrylate (PMMA), 13-µm silica, and 15-µm polystyrene (PS)] and selected the appropriate microbeads for a chronic ocular hypertensive model in terms of IOP elevation and adverse events. IOP changes were observed for 4 weeks after microbead injections. Axonal degeneration was assessed with transmission electron microscopic photographs and RGC loss was assessed with retrograde labeling. RESULTS: Seventy-eight rats were included. Three days after a single injection of microbeads, IOPs were increased by 24.0% by 7-µm PU microbeads, 101.8% by 17-µm PU microbeads, 56.6% by 7-µm PMMA microbeads, 22.0% by 15-µm PMMA microbeads, 153.0% by 13-µm silica microbeads, and 34.7% by 15-µm PS microbeads. 17-µm PU microbeads produced constant IOP elevation with good reproducibility (standard deviation of <6.5 mmHg). Silica injected eyes showed severe inflammation. Sustained IOP elevation by two injections of 17-µm PU microbeads resulted in a 42% axon loss and 36.5% RGC loss (p < 0.05, Mann-Whitney U test). CONCLUSIONS: PU microbead injections offer an applicable and versatile model for a chronic ocular hypertensive model in rats. Among several biomaterials, PU microbeads produced a more stable IOP elevation without adverse events. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 917~927 | - |
dc.relation.isPartOf | CURRENT EYE RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Anterior Chamber/drug effects | - |
dc.subject.MESH | Axons/pathology | - |
dc.subject.MESH | Chronic Disease | - |
dc.subject.MESH | Disease Models, Animal* | - |
dc.subject.MESH | Injections, Intraocular | - |
dc.subject.MESH | Intraocular Pressure/drug effects* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Microspheres* | - |
dc.subject.MESH | Ocular Hypertension/etiology* | - |
dc.subject.MESH | Ocular Hypertension/physiopathology | - |
dc.subject.MESH | Polymethyl Methacrylate/chemistry | - |
dc.subject.MESH | Polystyrenes/chemistry | - |
dc.subject.MESH | Polyurethanes/chemistry | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Retinal Ganglion Cells/pathology | - |
dc.subject.MESH | Silicon Dioxide/chemistry | - |
dc.subject.MESH | Suspensions/adverse effects | - |
dc.title | Chronic Ocular Hypertensive Rat Model using Microbead Injection: Comparison of Polyurethane, Polymethylmethacrylate, Silica and Polystyene Microbeads | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학) | - |
dc.contributor.googleauthor | Seungsoo Rho | - |
dc.contributor.googleauthor | Insung Park | - |
dc.contributor.googleauthor | Gong Je Seong | - |
dc.contributor.googleauthor | Naeun Lee | - |
dc.contributor.googleauthor | Chang-Kyu Lee | - |
dc.contributor.googleauthor | Samin Hong | - |
dc.contributor.googleauthor | Chan Yun Kim | - |
dc.identifier.doi | 10.3109/02713683.2014.884597 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04395 | - |
dc.contributor.localId | A01035 | - |
dc.contributor.localId | A01627 | - |
dc.contributor.localId | A01946 | - |
dc.contributor.localId | A02704 | - |
dc.relation.journalcode | J00665 | - |
dc.identifier.eissn | 1460-2202 | - |
dc.identifier.pmid | 24588104 | - |
dc.identifier.url | http://informahealthcare.com/doi/abs/10.3109/02713683.2014.884597 | - |
dc.subject.keyword | Chronic ocular hypertension | - |
dc.subject.keyword | microbead | - |
dc.subject.keyword | optic nerve | - |
dc.subject.keyword | polyurethane | - |
dc.subject.keyword | rat | - |
dc.contributor.alternativeName | Hong, Sa Min | - |
dc.contributor.alternativeName | Kim, Chan Yun | - |
dc.contributor.alternativeName | Park, In Sung | - |
dc.contributor.alternativeName | Seong, Gong Je | - |
dc.contributor.alternativeName | Lee, Na Eun | - |
dc.contributor.affiliatedAuthor | Hong, Sa Min | - |
dc.contributor.affiliatedAuthor | Kim, Chan Yun | - |
dc.contributor.affiliatedAuthor | Park, In Sung | - |
dc.contributor.affiliatedAuthor | Seong, Gong Je | - |
dc.contributor.affiliatedAuthor | Lee, Na Eun | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 39 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 917 | - |
dc.citation.endPage | 927 | - |
dc.identifier.bibliographicCitation | CURRENT EYE RESEARCH, Vol.39(9) : 917-927, 2014 | - |
dc.identifier.rimsid | 57092 | - |
dc.type.rims | ART | - |
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