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Linear Poly(ethylenimine) Cross-Linked by Methyl-β-Cyclodextrin for Gene Delivery.

Authors
 Wing-Fu Lai  ;  David W. Green  ;  Han-Sung Jung 
Citation
 CURRENT GENE THERAPY, Vol.14(4) : 258-268, 2014 
Journal Title
CURRENT GENE THERAPY
ISSN
 1566-5232 
Issue Date
2014
MeSH
Animals ; Cell Line, Tumor ; Chemistry Techniques, Synthetic ; Cross-Linking Reagents/chemistry ; Female ; Gene Transfer Techniques* ; Genetic Therapy/methods ; Humans ; Magnetic Resonance Spectroscopy ; Mice, Inbred C57BL ; Molecular Structure ; Molecular Weight ; Neoplasms/genetics ; Neoplasms/therapy ; Polyethyleneimine/chemistry* ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Spectroscopy, Fourier Transform Infrared ; Transfection/methods ; beta-Cyclodextrins/chemistry*
Keywords
Cancer ; cyclodextrin ; gene delivery ; gene therapy ; non-viral gene vector ; poly (ethylenimine)
Abstract
Poly(ethylenimine) (PEI) is a cationic polymer extensively exploited for non-viral gene delivery; however, its wide application has been impeded by its cytotoxicity. PEI can assume either a branched or linear configuration. Whereas branched PEI (bPEI) is more chemically reactive and can form smaller complexes with DNA under salt-containing conditions, lPEI is generally less toxic and exhibits higher transfection efficiency. In this study, we cross-linked low-molecularweight lPEI with methyl β-cyclodextrin (MβCD) to form MβCD-lPEI (MLP). The structure of MLP was successfully characterized by NMR, FT-IR, MALDI-TOF and elemental analysis. In the standard serum-free transfection environment, MLP could effectively transfect glioblastoma, melanoma and hepatocarcinoma cells. A high transfection efficiency was maintained in the presence of serum. Apart from its high transfection efficiency, MLP was found to have negligible cytotoxicity over a wide range of concentrations and to exhibit a low membrane disruptive capacity ex vivo. MLP warrants further development as a promising gene delivery system for future research.
Full Text
http://www.eurekaselect.com/122715/article
DOI
10.2174/1566523214666140612160042
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Lai, Wing Fu(여영부)
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/99624
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